Nyctotherus ovalis is a single-celled eukaryote that has hydrogen-producing mitochondria and lives in the hindgut of cockroaches. Like all members of the ciliate taxon, it has two types of nuclei, a micronucleus and a macronucleus. N. ovalis generates it
Genomic DNA fromNyctotheruswas separated on a 0.7% (w/v) native agarose gel and blotted onto a Hybond N+ membrane (Amersham) by downward alkaline southern blotting. The filter was pre-hybridized for 1 h at 65°C in hybridization solution (6× SSC, 5× Denhardts solution, and 0.5% SD...
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