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It was constructed through a PCR-mediated fusion of DNA fragments encoding RGS4 and GST, followed by cloning into BamHI/XbaI-cut pcDNA3. PCR-mediated site-directed mutagenesis was then used to introduce the M19A mutation. The plasmid pcDNA3-RGS16, expressing mouse RGS16 from the T7 ...
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Processed GST and residual GST-E2 fusion protein were removed by passing the sample through a second glutathione-Sepharose affinity column (30). Processed recombinant E2 contained in the resulting unadsorbed fraction was purified to apparent homogeneity by Mono Q anion exchange FPLC (9). The E2 ...
Despite its fundamental interest and applications, the experimental study of the USC regime is challenging. In this sense, superconducting quantum circuits9 are suitable systems to achieve large coupling strengths, despite some intrinsic limitations for most superconducting qubit types10. In fact, recent...
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Trouton's rule may and T0 if Δs would be the thus be formulated as: same for different fluids j at the boiling temperature T0j at reference pr∆ehssju=re Tp00.j∆Trso. uton's rule may thus be formulated as: (9) It has been argued from the principle of cohrjresTp0...