RT-qPCR analyses on the osteogenic differentiation from human iPS cells: an investigation of reference genesKensuke Okamura, Yusuke Inagaki, Takeshi K. Matsui, Masaya Matsubayashi, Tomoya Komeda, Munehiro Ogawa, Eiichiro Mori & Yasuhito Tanaka Scientific Reports volume 10, Article number: ...
Selection of suitable reference genes for RT-qPCR analyses in Cyanobacteria . PLoS ONE . 2012; 7 : e34983 doi: 10.1371/journal.pone.0034983Pinto F, Pacheco CC, Ferreira D, Moradas-Ferreira P, Tamagnini P. Selection of suitable reference genes for RT-qPCR analyses in cyanobacteria . PloS ...
reference gene with the lowest SD had the most stable expression. The geNorm, BestKeeper, Delta-Ct, and NormFinder analyses were performed using the ctrlGene [31] and NormqPCR [32] packages in R 4.2.1. Finally, RefFinder (http://www.leonxie.com/referencegene.php) was used to calculate t...
28,29. RT-qPCR analysis is highly sensitive, and its accuracy can be affected by RNA quantity, transcription efficiency, amplification efficiency and experimental procedures between samples. To avoid biases, normalization of gene expression is an essential step30. The most common practice ...
Relative gene expression analyses by RT-qPCR (reverse transcription-quantitative PCR) are highly dependent on the reference genes in normalizing the expres... L Liang,Z He,H Yu,... - 《Scientific Reports》 被引量: 0发表: 2020年 Selection and validation of reference genes for gene expression ...
Clinical relevance of DNA microarray analyses using archival formalin-fixed paraffin-embedded breast cancer specimens were validated using RT-qPCR, and two public databases were used to demonstrate the prognostic significance of the gene profiles generated from FFPE ... AM Sadi,DY Wang,Youngson, ...
Result tables of Cq and Cq analyses contain all calculated values for each gene/sample combination, e.g. mean values of Cq, standard errors and relative quantity or mean values of Cq, standard errors and fold change, respectively. Values in tables are rounded to two digits. Plots ...
(E). Data is collected for several potential reference genes. To select the best (most stable) genes usually one takes each proposed reference gene and performs statistical analysis comparing one gene at a time to that potential reference gene. I decided to perform analyses by comparing the ...
Identification of suitable candidate reference genes is crucial for RT-qPCR analyses of gene expression. Several methods have been developed but they ignore the compositional nature of RT-qPCR data. We propose here an equivalence test procedure to select appropriate reference genes and illustrate it on...
. Conditions for PCR amplifications have been described previously44,45. PCR products were cloned into the pCR4-TOPO vector (Invitrogen, Carlsbad, CA) and sequenced. After the identities of these reference genes were confirmed (Table S2), primers for the subsequent RT-qPCR analyses were ...