In RT-PCR methods for gene cloning and analysis [C]//Helen H Lee. Biotechniques Books. Natick:Eaton Publishing, USA,1998:305-319.Chenchik, A.; Zhu, Y.; Diatchenko, L.; Li, R.; Hill, J.; Siebert, P. RT-PCR Methods for Gene Cloning and Analysis. MA: BioTechniques Books; 1998....
Northern blot analysis and RNase protection assays are commonly used to study gene expression (1). However, both procedures require a significant amount of input RNA. In the case of the human malaria species, only Plasmodium falciparum can be grown in culture (2). This allows the production ...
用RNA/PCR方法来分析复合抗药性(MDR)10基因和 胸腺核苷酸合成酶(TS)基因,发现了这mRAN水平的增高.突变的RAS原癌基因的mRNA分 析(见参考文献25综术述)在癌症的诊断或预测方面具有诊断价值。mRNA分析比常规的 DNA基因组分析有优越之处。由于没有内含子序列干扰mRNA的扩增,因此可以仅用一 组引物就能够扩增H-,K-,...
当 只知一个内部序列时,只用一个基因特异性寡核苷酸引物,也可用PCR从稀有mRNA中 分离出cDNA。扩增cDNA的分析因使用本书其它章切有关PCR产物的直接序列分析步骤 而变得简单。将噬菌体(T7)启动了作为PCR引物的一部分同样可用于序列分析,因为 PCR终产物中有大量的群体特异的产物。
2007. Fast and accurate quantitative analysis of cytokine gene expression in human neutrophils by reverse transcription real-time PCR. Methods Mol. Biol 412... N Tamassia,MA Cassatella,F Bazzoni - 《Methods Mol Biol》 被引量: 32发表: 2007年 Gene Expression Profiling during Wilting in Chickpea...
本产品配有单独的ROX内参染料,使得该试剂可应用于所有RealTime PCR 机型,包括:ABI 7000/7700/7900HT, 7300/7500/7500 Fast Real-Time PCR(Applied Biosystems);Mx3000P(Stratagene);LightCycler(Roche Diagnostics) ;Opticon2、MyiQ 2、CFX96 Real-Time PCR(Bio-Rad&MJ);Line-Gene(Bioer,杭州博日)等。
一步法荧光定量RT-PCR试剂盒(探针法)产品规格: 50μl×50次 发货周期: 1~3天 一步法荧光定量RT-PCR试剂盒(探针法) 本试剂盒是采用探针法(TaqMan,Molecular Beacon等)进行Real Time One Step qRT-PCR的专用试剂。使用本制品进行Real Time One Step qRT-PCR反应可在同一反应管内连续进行,操作简单,并能有效防...
For quantification of gene-specific mRNA, quantitative real-time RT-PCR has become one of the most frequently used methods over the last few years. This article focuses on the issue of real-time PCR data analysis and its mathematical background, offering a general concept for efficient, fast ...
Fernando Basso1, Bárbara Andrade Dias Brito da Cunha1, Adilson Kenji Kobayashi1 & Hugo Bruno Correa Molinari1 Real-time PCR (RT-qPCR) expression analysis is a powerful analytical technique, but reliable results depend on the use of stable reference genes for proper ...
D Porombka,W Baumg?Rtner,C Herden - 《Journal of Virological Methods》 被引量: 17发表: 2008年 The use of TaqMan RT-PCR assays for semiquantitative analysis of gene expression in CNS tissues and disease models TaqMan reverse transcription polymerase chain reaction (RT-PCR) is a recently dev...