Core costs and processing time of extraction-free RT-LAMP are similar to RT-PCR workflows (Table 4), but can be done without expensive machines or specialized labs, by trained non-specialists with oversight and support. This frees nurses and other front-line staff for less technical duties ...
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In the present study, the genotype of each animal was assessed twice using PCR and by sequencing genomic DNA isolated from tail tissue both before and after each experiment. The age of the mice used in this study is indicated in each section of the online methods. All efforts were made to...
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Total RNA (2 µg) was reverse transcribed using the MLV-RT kit from Invitrogen and 1/20 of the products were used for PCR amplification. We performed re- al-time PCR analysis of Prrt2 mRNA expression using a SYBR Green I Premix Ex Taq Kit (Takara, Shiga, Japan) and an ABI Prism...
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For International Flights:Kindly confirm with your chosen airline if they require any specific labs for your RT-PCR test prior booking on Klook For any questions or concerns prior booking, you may reach the partnered lab via mobile: 09178057331, 09171465324, or 09171425325 ...
Abbreviations RT-qPCR Real-Time PCR G-layer Gelatinous layer References 1. Wu, Z.-J.; Tian, C.; Jiang, Q.; Li, X.-H.; Zhuang, J. Selection of suitable reference genes for qRT-PCR normalization during leaf development and hormonal stimuli in tea plant (Camellia sinensis). Sci. Rep...