(tagged with eGFP) mRNA overexpressed 6 daf medaka embryos. Since we observed differential but significantDmyknockdown dependent down-regulation ofGsdfandSox9a2and up-regulation ofRspo1, the potential downstream targets forDmy, these genes were chosen as candidates for ChIP analysis. The primers were...
(fihveerFe 0affitsehr gave rise to considered viable progeny, complete AS construct was integrated into the genome of two spec- as founder fish), as evidenced by the amplification of the transgene sequence (using a vector specific forward primer and a Dmy fragment specific reverse primer) from...