Plasmid pBW, containing the rrnB P1P2 region spanning from –235 to +237 bp (using P1 as a reference) was constructed by removing a 370-bp rrnB T1T2 terminator fragment from plasmid pPS1 (26) and ligating it to an EcoRI and HindIII fragment containing the rrnB P1P2 region from –235...