HTERT RPE-1细胞系|人视网膜色素上皮细胞可以用于谷胱甘肽在微波辐射致hTERT-RPE1细胞基因转录中的作用 对HTERT RPE-1细胞系|人视网膜色素上皮细胞细胞和添加5 0μmol·L-1谷胱甘肽(GSH)培养2 4h后的hTERT RPE1细胞进行2 4 5 0MHz,1 0mW·cm-2的微波辐射,并就有关细胞应激和凋亡基因的转录水平进行比较研究。
中文名称:hTERT RPE-1 Cells:人视网膜色素上皮细胞系英文名称:hTERT RPE-1 Cells 保存条件:低温避光纯度规格:1×10(6)cells/T25培养瓶 产品类别:实验试剂 "hTERT RPE-1 Cells:人视网膜色素上皮细胞系 细胞形态特性:详见细胞说明书 65039-04-5 细胞生长特性:贴壁或悬浮,详见细胞说明书部分 ...
To better understand the early effects of MYCN activation in a neural-crest lineage context, we profiled the transcriptome of immortalized human retina pigment epithelial cells with inducible MYCN activation. Gene signatures associated with elevated MYC/MYCN activity were induced after 24h of MYCN ...
In AMD, retinal homeostasis is compromised due to the dysfunction of retinal pigment epithelium (RPE) cells. To investigate the impact of endogenous FH loss on RPE cell balance, we silencedin human hTERT-RPE1 cells. FH reduction led to accumulation of C3, at both RNA and protein level and ...
However, the role of m6A methylation in the EMT of human telomerase reverse transcriptase (hTERT) retinal pigmental epithelium (RPE)-1 cells has not been clarified. Here, we extracted RNA from RPE cells subjected to 0 or 20 ng/mL TGF-β2 for 72 h and identified differentially methylated ...
The results obtained from primary RPE cells were replicated using the hTERT-RPE cell line. RNAseq expression analysis of hTERT-RPE cells bound to FHL-1 showed an increased expression of the heat-shock protein genes HSPA6, CRYAB, HSPA1A and HSPA1B when compared to cells bound to fibronectin ...
Methods. Reverse transcription coupled to polymerase chain reaction (RT-PCR) was performed after total RNA extraction from different RPE (ARPE-19, monkey, hTERT-RP1 and D407) and non-RPE (COS-7, HeLa, HepG2 and HS27) cell lines. Promoter activity was assayed by transient transfection of ...
However, it is also known that PBM can induce elevated levels of nitric oxide (NO) in cells, which is thought to occur by release of NO bound to cytochrome-c oxidase (COX). NO is a powerful signaling molecule involved in a host of biological responses; however, the mechanisms of NO ...
However, it is also known that PBM can induce elevated levels of nitric oxide (NO) in cells, which is thought to occur by release of NO bound to cytochrome-c oxidase (COX). NO is a powerful signaling molecule involved in a host of biological responses; however, the mechanisms of NO ...
Methods : We used cultured retinal pigment epithelial (RPE) cells (hTERT-RPE1), and the mitochondria isolated from them (Qiagen kit) to investigate the metabolic effects and the primary chromophores for low irradiance light at various wavelengths. Photo-oxidation occurred at wavelengths expected to ...