减数分裂过程中会产生大量的程序性DNA双链断裂,使得细胞内产生ssDNA-RPA纤丝结构的概率显著增加。但目前关于如何移除减数分裂细胞中ssDNA-RPA纤丝结构的机制还不清楚。 近日,张亮然教授团队联合王顺心教授团队针对上述问题在Nucleic Acids Research发表了“Dna2 removes toxic ssDNA-RPA filaments generated from meiotic re...
(2002). Rad52 protein asso- ciates with RPA-ssDNA to accelerate Rad51-mediated displace- ment of RPA and presynaptic complex formation. J. Biol. Chem. 277, 31663-31672.Sugiyama,T. and Kowalczykowski,S.C. (2002) Rad52 protein associates with replication protein A (RPA)-single-stranded ...
因此，Pra扩增不是一种不对称扩增方法。关于生成ssDNA的问题，Pra扩增本身并不能直接生成单链（ss）DNA。
g, Co-assembly of ssDNA into RPA droplets. Cy3-labeled purified RPA was incubated with equimolar 17-nucleotide-oligomer FAM-labeled ssDNA. Two representative example images of RPA-ssDNA droplets are shown. h, RPA-ssDNA droplets maintain liquid properties and undergo fusion. Purified RPA was ...
The eukaryotic single-stranded DNA (ssDNA)-binding protein Replication Protein A (RPA) plays a crucial role in various DNA metabolic pathways, including DNA replication and repair, by dynamically associating with ssDNA. While the binding of a single RPA
Importantly, PRP19 mutants that are unable to bind RPA or function as an E3 ligase failed to support the ATR response, suggesting that the full activation of ATR is driven by a ubiquitylation-mediated circuitry orchestrated by RPA-ssDNA and PRP19. Our results show that an RNA-processing ...
(ssDNA). The strong RPA affinity for ssDNA, KAbetween 10−9 − 10−10M, is characterized by a low cooperativity with minor variation for changes on the nucleotide sequence. Recently, new data on RPA interactions was reported, including the binding free energy of the complex RPA70AB ...
RPA nucleic acid-binding properties of IFI16-HIN200 Our results indicate that IFI16-HIN200 is an RPA-like, OB-fold, nucleic acid-binding protein that binds to ssDNA with higher affinity than to dsDNA,... H Yan,K Dalal,BK Hon,... - 《Biochimica Et Biophysica Acta》 被引量: 68发表:...
由此产生的ssDNA被RPA快速结合,进一步刺激DNA切除。作为DNA损伤反应(DDR)的一部分,RPA在切除期间被磷酸化。值得注意的是,在细胞实验和体外实验中,pRPA通过抑制BLM解旋酶来抑制DNA切除。 pRPA抑制BLM在DNA末端的起始,促进固有解旋酶的strand-switch活性。这些发现证实了pRPA在DNA切除和DNA损伤反应之间存在一个反馈回路...
At stalled forks, RPA-coated single-stranded DNA (ssDNA) activates the ATR kinase and directs fork remodeling, 2 key early events of the replication stress response. RFWD3, a recently described Fanconi anemia (FA) ubiquitin ligase, associates with RPA and promotes i...