LARP1-mRNA levels relative to 18S ribosomal RNA (P = 0.012, Student's t-test). (c) LARP1 immunostaining (brown) of normal cervical epithelium (N12), cervical intraepithelial neoplasia (CIN; N35) and invasive cervical SCC (N36), counterstained with haematoxylin. (d) LARP1 cytoplasmic scores...
*p < 0.05,***p < 0.001 (significantly different from TERT-pSUPER cells using a student's t-test). Full size image Normal adherent cells are strongly dependent on adhesion to extracellular matrix for cell proliferation and undergo apoptosis if they are detached from the substratum, in a ...
Data were analyzed by Student's t test and presented as means ± SEM. ∗∗p < 0.01, ∗∗∗p < 0.001. See also Figure S1. Download: Download high-res image (1MB) Download: Download full-size image Figure 2. Characterization of WT, Sfrp1+/−, and Sfrp1−/− Mouse ...
To test whether SSRP1 is required in serum-induced EGR1 expression, we conducted a set of serum stimulation experiments. Under normal or serum starvation conditions, the EGR1 protein level was detected at extremely low levels (Fig. 4A). After 20% serum stimulation for 1.5 h, the EGR1 ...
the Cataract (control) group by one-way ANOVA, followed by Dunnett’s test. Abbreviations: POAG = primary open-angle glaucoma, CACG = chronic angle-closure glaucoma, PACS = primary angle-closure suspects, AACG = acute angle-closure glaucoma Full size image There were ...
Form liquid Purification Immunogen affinity purified Purity ≥95% as determined by SDS-PAGE Clonality polyclonal Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.) Immunogen Immunogen structure specific recognition pro...
Full size image LARP1 enhances spherosome formation To investigate the effect of LARP1 on cell survival in anchorage-independent conditions, we then cultured LARP1-overexpressing cells in ultra-low attachment plates. Under these conditions, single cells form floating colonies termed spherosomes. LARP1...
6R). These analyses indicate that the GTP- and GDP-locked form of Arl14 is preferentially located at the plasma membrane or endosomes, respectively. We then used a permeabilized cell assay to test whether membrane recruitment of these Arf proteins depends on GTP. HeLa cells were permeabilized ...
The student’s t-test was used to calculate the significance of data, where *p < 0.05 and **p < 0.01. Apical spikelets of apa1331 had higher level of ROS Excessive ROS accumulation could happen either due to genetic factors or in response to biotic and abiotic stresses [67]. These ROS ...
If Sfrp1 or Sfrp2 is a putative suppressor of Wnt/β-catenin activity in presumptive lens region, it would be expected that TCF/Lef-lacZ activity would be enhanced inSfrpDKO lenses. To test this we examined β-gal activity in lens placode by generatingSfrp1;Sfrp2;TCF/Lef-lacZmice. As...