JKBioHigh5TM系列Rosetta(DE3) 感受态细胞具有氯霉素抗性,补充大肠杆菌缺乏的6 种稀有密码子(AUA,AGG, AGA, CUA,CCC, GGA) 对应的tRNA,提高外源基因,尤其是真核基因在原核系统中的表达水平,该菌株染色体整合了λ噬菌体DE3区(DE3区含有T7噬菌体RNA聚合酶),同时表达T7 RNA聚合酶和大肠杆菌RNA聚合酶,可用于pET系列...
Rosetta(DE3)pLysS 感受态细胞0.1mL×10品牌AntibioticAgarNO.2 MMO-MUG 100(g) incubation media MMO-MUG 100(g) 酵母提取物琼脂 Yeast Extract Agar 250克 水中细菌总数的检测 快速硫化氢(H2S)试验琼脂250用于弯曲杆菌的硫化氢试验(GB标准)incubationmedia快速硫化氢(H2S)试验琼脂250用于弯曲杆菌的硫化氢试验(GB标准...
Rosetta(DE3)感受态细胞由特殊工艺制作,pUC19质粒检测转化效率高达108cfu/μg DNA。 Rosetta(DE3)热激感受态细胞操作方法 1. Rosetta(DE3)感受态细胞从-80℃拿出,迅速插入冰中,5分钟后待菌块融化,加入目的DNA(质粒或连接产物)并用手拨打EP管底轻轻混匀(避免用枪吸打),冰中静置25分钟。
3. Inoculate 50 ml of LB medium containing the appropriate antibiotic with 0.5 ml of the overnight culture prepared in step 2(use the 500 ml triangular flask as the container would be better). 4. Incubate with shaking at 150 rpm at 37℃ until the OD 600 reaches 0.5-0.8. (0.6 recommend...
1. Inoculate a single colony from a freshly streaked plate into 5 ml of LB medium containing the appropriate antibiotic for the plasmid and host strain. 2. Incubate with shaking at 200 rpm at 37℃ overnight. 3. Inoculate 50 ml of LB medium containing the appropriate antibiotic with 0.5 ml...
3. Inoculate 50 ml of LB medium containing the appropriate antibiotic with 0.5 ml of the overnight culture prepared in step 2(use the 500 ml triangular flask as the container would be better). 4. Incubate with shaking at 150 rpm at 37℃ until the OD 600 reaches 0.5-0.8. (0.6 recommend...
Rosetta-gami(DE3)pLysS热激感受态细胞 Rosetta-gami(DE3)pLysS: 100μl/支 pUC19 ( control vector, 10pg/μl ): 10μl 保存条件(保质期): -80 ℃(6个月) Rosetta-gami(DE3)pLysS热激感受态细胞 基因型 Δ (ara-le
Rosetta-gami 2(DE3) Chemically Competent Cell说明书 产品货号: JK-G87019 保存条件: -80℃ 产品规格: 10×100μl50×100μl 产品介绍 基因型 D(ara-leu)7697 DlacX74 DphoA PvuII phoR araD139 ahpC galE galK r
1. Rosetta-gami B(DE3)菌株感受态细胞从-80℃拿出,迅速插入冰中,5分钟后待菌块融化,加入目的质粒并用手拨打EP管底混匀,冰中静置25分钟。 2. 42℃水浴热激45秒,迅速放回冰上并静置2分钟,晃动会降低转化效率。 3. 向离心管中加入700 μl不含抗生素的无菌培养基 (2YT 或 LB),混匀后37℃, 200 rpm ...
FIG. 4 shows the validation of expression of miRNAs predicted in EBV (Epstein's Barr Virus) miRNAs; expression validation. Three cell line were tested. Two were freshly infected normal B-cells (PBMC-1/2-EBV), and one EBV-transformed cell line (B-95-8). The 3 cell lines exhibit the s...