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rhizogenes strain ATCC 15834 harboring a binary vector carrying the reporter gene EGFP. This gene served as an indicator of successful HR formation. Several experimental factors were investigated for hairy root formation: rose explant types, wounding via sonica- tion, different R. rhizogenes strains,...
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vector combinations shown were introduced into yeast cells. The wild-type fragment (−1003 to −1396 bp) of theRhLOX4promoter was used in theLacZreporter vector.eTransrepression activity of theRhLOX4promoter by RhHB1 inN. benthamianaleaves. The promoter region (0 to −1436 bp) of...
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The slides were then washed and mounted on coverslips with Vectashield mounting medium (Vector, Vector Laboratories, CA, USA). All of the sections were observed and photographed under a fluorescence microscope with a blue excitation light (LSM710, Zeiss, Thornwood, NY, USA). 2.16. Glial ...
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