using the recombinase mediated cassette exchange technology to integrate the dCas9-VPR gene containing cassette into theROSA26locus (Fig.1A). The cloned targeting vector contained a NeoR cassette, the human CAG promoter, and a translation interrupting LSL cassette linked with thedCas9-VPRgene ...
The cloned targeting vector contained a NeoR cassette, the human CAG promoter, and a translation interrupting LSL cassette linked with the dCas9-VPR gene fused to a self-cleaving P2A sequence and Egfp gene (Fig. 1A). Characterization of Cre‑dependent and tissue‑specific dCas9‑VPR ...
Using a different Lrig1 knock-in mouse model (i.e., eGFP-IRES-CreERT2 cassette integrated into exon 1 of the Lrig1 gene), they reported that only a small population of the Lrig1-positive cells in the glandular epithelium was proliferating, leading to the suggestion that Lrig1 was ...
LSL-Cas9-Rosa26TV catalog : 61408 more info or order: Addgene product webpage citations: 7 Reference Gierus L, Birand A, Bunting M, Godahewa G, Piltz S, Oh K,et al. Leveraging a natural murine meiotic drive to suppress invasive populations. Proc Natl Acad Sci U S A. 2022;119:e...
Conclusions : The NOD.129(Cg)-Gt(ROSA)26Sortm1.1(CAG-cas9*,-EGFP)Fezh/J mouse would be a suitable model for optimising Cas9 knockdown in retinal ganglion cells or M眉ller cells, but may be a poor model for photoreceptor targets. The apparent lack of both Cas9 and EGFP in this model...
求助：请问哪位大神有129-Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh/J这个小鼠？实验急用，谢谢。
Rosa26-LsL-cas9-EGFP小鼠 Rosa26-LsL-cas9-EGFP是绿色荧光基因编辑工具小鼠。该小鼠与全身表达或组织特异表达Cre重组酶的品系交配后,将会在表达Cre的细胞中删除STOP元件,开启Cas9和EGFP的表达。可用于活体动物基因编辑的研究,也可当做cre工具鼠的报告小鼠。
The cloned targeting vector contained a NeoR cassette, the human CAG promoter, and a translation interrupting LSL cassette linked with the dCas9-VPR gene fused to a self-cleaving P2A sequence and Egfp gene (Fig. 1A). Characterization of Cre‑dependent and tissue‑specific dCas9‑VPR ...