SD-ROSA26-LSL-tdTomato大鼠(产品编号:CR003)是一种条件性激活红色荧光的Reporter模型。在该模型中,tdTomato基因表达元件被整合到ROSA26安全位点,并受CAG启动子的调控。正常情况下,其表达会被上游的LSL元件阻断,但可以通过Cre重组酶去除LSL元件以激活tdTomato的表达。SD-ROSA26-LSL-tdTomato大鼠在Cre重组酶的作用下...
其中,EGFP和tdTomato等荧光蛋白因其亮度高、稳定性好以及光谱特征与兼容性良好等优点,在小鼠体内得到了广泛应用[3-4]。结合Cre/LoxP重组酶系统,Reporter小鼠极大地推动了细胞生物学的研究。然而,在神经生物学、行为学、高阶脑功能以及器官发育或移植等特殊领域,利用Reporter小鼠作为研究模型仍存在一定的局限性[5]。
Because the administration of a high dose of tamoxifen to Lgr5-DTR-GFP mice led to the expression of Lgr5 and Gpr30 in the upper mucosal region and thus raised the question of selective chief cell labeling, the authors replaced the tamoxifen-dependent Cre/LoxP system with a tamoxifen-...
Transient transfection of pRosa26-tdTomato and pCMV-tdTomato vectors was performed in the indicated cell lines. (D) A diagram for TALEN-me- diated knock-in of Neo-polyA-iEGFP into the pRosa26 locus. Grey triangles, wild-type (WT) loxP; white triangles, loxP2272 site; SA, splice ...
The loxP and mutant loxP2272 sites were arranged to flank the tdTomato and iEGFP genes. (Fig. 2A) Microinjection and embryo transfer. Sexually matured (6–18 months) New Zealand White (NZW) female rabbits were superovulated by subcutaneous injection of follicle-stimulating hormone (FSH,...
其中,EGFP和tdTomato等荧光蛋白因其亮度高、稳定性好以及光谱特征与兼容性良好等优点,在小鼠体内得到了广泛应用[3-4]。结合Cre/LoxP重组酶系统,Reporter小鼠极大地推动了细胞生物学的研究。然而,在神经生物学、行为学、高阶脑功能以及器官发育或移植等特殊领域,利用Reporter小鼠作为研究模型仍存在一定的局限性[5]。