在无Tamoxifen诱导时,Cre-ERT2处于无活性状态;Tamoxifen诱导后,其代谢产物4-OHT与ERT结合,使Cre-ERT2进核发挥Cre重组酶活性。 🌈 Rosa26-LSL-reporter小鼠:通过在Rosa26基因终止密码子前后敲入LSL和tdTomato,使得特定细胞或组织类群在Tamoxifen注射后被标记为红色。这样,研究人员就可以直观地观察到特定基因的表达情况...
C57BL/6-Rosa26/tdTomato 小鼠,C57BL/6-Rosa26/tdTomato小鼠•专业名称:B6-Gt(Rosa26 Sor–TdTomato)•毛色:黑色•基因型:loxP-STOP-loxP-TdTomato在Rosa26位点的定点转基因•特征:与组织特异性表达Cre重组酶的小鼠交配
The encoded tdTomato transgene was highly expressed in all tissues evaluated. Targeted integration was verified by PCR analyses, Southern blot, and germ-line transmission. Function of the ROSA26-CAG-LoxPtdTomatoStopLoxPEGFP (ROSA-TG) Cre-reporter was confirmed in primary cells following Cre ...
Once in the nucleus, it can recombine the loxP-flanking the GHR, allowing for the generation of GHRKO mice at specific ages (right diagram). While genetically modified mouse lines are commonly used in research, their origin is often taken for granted or forgotten. However, evaluating their ...