Conclusions : The NOD.129(Cg)-Gt(ROSA)26Sortm1.1(CAG-cas9*,-EGFP)Fezh/J mouse would be a suitable model for optimising Cas9 knockdown in retinal ganglion cells or M眉ller cells, but may be a poor model for photoreceptor targets. The apparent lack of both Cas9 and EGFP in this model...
We generated the Cre-dependent CRISPR activation mouse line, termed Rosa26-LSL-dCas9-VPR, using the recombinase mediated cassette exchange technology to integrate the dCas9-VPR gene containing cassette into theROSA26locus (Fig.1A). The cloned targeting vector contained a NeoR cassette, the human ...
Using transgenic mice harboring the eR1-mHSP68-EGFP transgene, where the eR1 enhancer drives EGFP, Matsuo et al. observed strong eR1 activity in proliferating isthmus stem cells of the stomach corpus and in a small number of chief cells at the base of the gland [44]. Furthermore, ...
求助：请问哪位大神有129-Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh/J这个小鼠？实验急用，谢谢。
LSL-Cas9-Rosa26TV catalog : 61408 more info or order: Addgene product webpage citations: 7 Reference Gierus L, Birand A, Bunting M, Godahewa G, Piltz S, Oh K,et al. Leveraging a natural murine meiotic drive to suppress invasive populations. Proc Natl Acad Sci U S A. 2022;119:e...
我们高效地将绿色荧光蛋白EGFP替换为红色荧光蛋白tdDIMER,证实bRosa26位点可以介导高效的RMCE实现基因替换.本研究将三种广谱型启动子启动EGFP表达的打靶载体高效地定点整合在bRosa26位点上,在细胞水平上检测到EGFP均能持续稳定表达,且其表达对bRosa26上下游邻近基因的表达未造成显著干扰.其中CAG启动子启动EGFP的表达活性...
The cloned targeting vector contained a NeoR cassette, the human CAG promoter, and a translation interrupting LSL cassette linked with the dCas9-VPR gene fused to a self-cleaving P2A sequence and Egfp gene (Fig. 1A). Characterization of Cre‑dependent and tissue‑specific dCas9‑VPR ...
Rosa26-LsL-cas9-EGFP小鼠 Rosa26-LsL-cas9-EGFP是绿色荧光基因编辑工具小鼠。该小鼠与全身表达或组织特异表达Cre重组酶的品系交配后,将会在表达Cre的细胞中删除STOP元件,开启Cas9和EGFP的表达。可用于活体动物基因编辑的研究,也可当做cre工具鼠的报告小鼠。
The cloned targeting vector contained a NeoR cassette, the human CAG promoter, and a translation interrupting LSL cassette linked with the dCas9-VPR gene fused to a self-cleaving P2A sequence and Egfp gene (Fig. 1A). Characterization of Cre‑dependent and tissue‑specific dCas9‑VPR ...
求助：请问哪位大神有129-Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh/J这个小鼠？实验急用，谢谢。