dihydroethidium (DHE), and mitochondrial-targeted DHE (mitoSOX) are the most popular ones. The diacetate form of DCFH (DCFH-DA) is a cell-permeable form that allows ester loading of the dye, resulting in intracellular accumulation of the nonfluorescent DCFH. In the presence of H2O2and other...
Tunneling nanotubes (TNTs) are novel cell–cell communication that plays a crucial role in pathology and physiology. However, the relationship between TNTs and nanomaterials neurotoxicity remains unclear. Here, three types of commonly used engineered nanomaterials, namely cobalt nanoparticles (CoNPs), ...
Full size image However, a growing body of evidence supports the view that antioxidant activities are essential for tumorigenesis. It has been recently reported that targets of the Nrf2 gene, such as HMOX1, facilitate cancer development because they counteract the effect of oxidative stress in trans...
Hajam YA, Rani R, Ganie SY, Sheikh TA, Javaid D, Qadri SS, Reshi MS (2022) Oxidative stress in human pathology and aging: molecular mechanisms and perspectives. Cells 11(3):552 ArticleCASGoogle Scholar Hartwig A (2013) Metal interaction with redox regulation: an integrating concept in me...
‘Reactive oxygen species’ (ROS) is an umbrella term for an array of derivatives of molecular oxygen that occur as a normal attribute of aerobic life. Elevated formation of the different ROS leads to molecular damage, denoted as ‘oxidative distress’.
Department of Pathology, University of Zagreb School of Medicine, 10 000 Zagreb, Croatia, Clinical Department of Pathology and Cytology, University Hospital Center Zagreb, 10 000 Zagreb, Croatia. View Full Text PDF Images No reagents have been identified in images within this publication yet ...
Sign in to download full-size image Figure 29.6. Japanese encephalitis virus (JEV) and West Nile virus (WNV) transmission cycles. With both viruses, the enzootic cycles are maintained by mosquito vectors infecting amplifying hosts (WNV—predominantly birds; JEV—birds and swine). Humans, horses,...
Download:Download full-size image Fig. 4. RE as a function of substrate addition in non-stressed mitochondria. The experiment was carried out with MCB-loaded [20 μM] mitochondria as described inFig. 3. The relative abundance of NAD(P)H (%) and GSH (mM) and H2O2emission were monitored...
Full size image To demonstrate that elevated levels of ROS can directly promote IDD, we administered subcutaneous injections of 50 µL of 2.5% H2O2or PBS (control) near the lumbar discs of mice every five days for 12 weeks (Fig.1G). At the end of the experiment, H&E staining revealed...
Full size image Many lines of evidence suggest that RSV can activate the NLRP3 inflammasome and induce inflammasome-related airway inflammation, even pyroptosis [70,76,77], similar to other RNA viruses [73]. As we mentioned before, RSV can induce excessive production of ROS [15,54], which ...