TdRFP+)表达tomato red+,而正表达FOXP3的Tregs(functional Tregs, TdRFP+GFP+)表达GFP+,以此来区分Tregs的功能;结果发现MPTP处理的小鼠脾脏中TdRFP+/ TdRFP+GFP+ Tregs细胞增加(图2G),提示Tregs向其它CD4 T细胞过渡。类似
???GFP GFP PCR Rorc t 法鉴定突变小鼠子代?GFP 基因型 陈嵘，阿彩岭，周英，樊翌明 祎（广东医学院附属医院皮肤科，广东湛江）524001ＰＣＲ－ｂａｓｅｄｇｅｎｏｔｙｐｅｉｄｅｎｔｉｆｉｃａｔｉｏｎｏｆｆｉｌｉａｌｇｅｎｅｒａｔｉｏｎｉｎＲｏｒｃｔｍｕｔａｔｉｏｎｍｉｃｅ ?ＧＦＰCHEN Rong-yi,E Cai-ling,ZHOU ...
第3O卷第6期2012年12月广东医学院学报JOI瓜NALOFGUANGD0NGMEDICALCOLLEGEV_0l_30No.6Dec.2012603PCR法鉴定R0rcGFP突变..
小鼠核受体基因突变聚合酶链式反应目的 探讨PCR(聚合酶链式反应)法鉴定RorcγtGFP突变小鼠子代基因型的可行性.方法 Rorcγt(-/-)基因型雄性与Rorcγt(-/+)基因型雌性小鼠产生的6只新生小鼠,取尾部组织提取DNA,进行PCR扩增及琼脂糖凝胶电泳检测PCR产物,根据产物片段大小鉴定新生小鼠基因型.结果 3只小鼠PCR产物具有...
Objective To investigate the feasibility of PCR in the genotype identification of RorcγtGFP mutation mouse offspring. Methods The genome DNA was extracted from the tails of six newborn mice produced by a male Rorcγt(-/-) mouse and a female Rorcγt(-/+) mouse. Rorcγt gene fragments we...
After isolation cells were pooled, and GFP+ cells were sorted using a BD FACSAria™ III sorter with a 85-micron nozzle and a threshold of 3000 events/second. Cells were sorted into Eppendorf tubes containing 1ml FACS buffer, followed by centrifugation without brake. Cells were counted and ...
The control or c-Maf-RV-GFP plasmid was transfected into 293T cells together with the pCL-ECO packaging plasmid using calcium phosphate. The supernatant containing retroviruses were harvested 48 hours post transfection. Naive CD4+ T cells were sorted and activated under Th0 condition for 24 hours...
GFP GFP PCR Rorc t 法鉴定突变⼩⿏⼦代?GFP 基因型 陈嵘，阿彩岭，周英，樊翌明 祎（⼴东医学院附属医院⽪肤科，⼴东湛江）524001ＰＣＲ－ｂａｓｅｄｇｅｎｏｔｙｐｅｉｄｅｎｔｉｆｉｃａｔｉｏｎｏｆｆｉｌｉａｌｇｅｎｅｒａｔｉｏｎｉｎＲｏｒｃｔｍｕｔａｔｉｏｎｍｉｃｅ ＧＦＰCHEN Rong-yi,E Cai-ling...
GFP GFP PCRRorct法鉴定突变小鼠子代? GFP 基因型 陈嵘,阿彩岭,周英,樊翌明 祎 (广东医学院附属医院皮肤科,广东湛江)524001 PCR-basedgenotypeidentificationoffilialgenerationinRorctmutationmice? GFP CHENRong-yi,ECai-ling,ZHOUYing,FANYi-ming(DepartmentofDermatology,AffiliatedHospitalofGuangdong MedicalCollege,Zhanj...
结果 3只小鼠PCR产物具有241bp片段,为Rorcγt(-/-)基因型;其余3只具有174bp及241bp片段,为Rorcγt(-/+)基因型。结论 PCR是检测RorcγtGFP突变品系小鼠基因型的简便、可靠方陈嵘祎广东医学院附属医院阿彩岭广东医学院附属医院周英广东医学院附属医院樊翌明广东医学院附属医院广东医学院学报...