Finally, labeled cRNA with cyanine-3-CTP was hybridized onto Rice Gene Expression Microarrays, 4x44K, containing 43,803 probes (Agilent; G2519F-015241). After washing, the arrays were scanned with an Agilent Surescan microarray scanner. Feature Extraction software (version 11.5.1.1; Agilent ...
stage. Root–shoot ratio is also affected by the nutritional status of plants. Shortage of phosphate supply was found to increase mainly the ratio of root length per weight of plants (Fist, 1987;Jungket al., 1990). The regulating mechanism is root cell elongation (Anuradha and Narayanan, ...
Following in vivo injection of radioactively labeled amino acids, newly synthesized proteins are predominantly associated with the monomeric ribosomes. Ribonuclease has been identified in the components of the cell-free system whose activity is lower than that of a similar system prepared from mammalian...
The length and number of root hairs are regulated by genetic factors. Their formation follows one of three patterns: 1)random, any cell of the epidermis being capable of root-hair development; 2)alternative, morphologically different cells alternating along longitudinal epidermal cell files, with asy...
in each genotype at 12 days after withholding watering (n = 8). Three independent experiments showed similar results for (dande). Results are presented as mean ± stand error of the mean (SEM). ThePvalues between each mutant and Col-0 were labeled above each bar (two-sided Stude...
with genes with significant plant gene ontology (GO) terms are labeled accordingly. Scatter plots illustrating the hub OTUs interacting with functional genes enriched in specific plant GO terms in the lateral root (C). Different color dots indicate different plant GO terms, referring to the network...
The auxin transport assay with 3H-labeled indoleacetic acid (3H-IAA) showed efficient shootward auxin transport from the root tip of P. taeda that was sensitive to the N-1-naphthylphthalamic acid (NPA), an established inhibitor of auxin transport44 (Supplementary Fig. 10d, e). The shootward...
generates the root-hair cell and non-root-hair cell [51]. TheA. thalianaroot-hair patterning type is shared by all species of theBrassicaegenus [29], whereas the rice pattern (i.e., a short cell bearing a root hair alternating on the same file with a long, non-root-hair cell) is ...
a Fluorescence microscopy of GFP-labeled Rhodanobacter sp. Root179 (Root179::GFP) colonizing A. thaliana root surface at 14 dpi. Scale: 20 µm, Arrows: accumulation of bacteria in grooves between epi- dermal cells, Asterisk: root hair emergence site. This experiment was done only one ...
(Invitrogen) and 10 μg of total RNA were loaded in each lane of a denaturing 17% polyacrylamide gel, size-separated by electrophoresis and transferred to a nylon membrane. The following hybridizations were performed as described in Sunkar and Zhu46using32P-labeled probes complementary to miR...