Furthermore, the year 2022 was compared to 2020, the latter having been characterised by temperatures in line with the last two-decades. The heatwaves do not significantly affect the onset time and the duration of sea breeze, while its cooling effect intensifies during the heatwaves. The urban...
A barcoded Nanopore library was built for the baseline virome using Rapid Barcoding Sequencing kit (SQK-RBK004) (Oxford Nanopore Technologies, Oxford, UK) and sequenced on the MinION platform using an R9.4 flow cell (Oxford Nanopore Technologies, Oxford, UK). The Ligation Sequencing kit (SQK-...
of the blood virome and showed remarkable diversity. In total, we assembled 332 complete or near-complete anellovirus genomes, 50 of which could be considered new species. HPgV was much less frequent, but we, nevertheless, recovered 17 different isolates that we subsequently used for characterizin...
The vibration effects on the Torre della Moletta and the ruins of the Circus Maximus in Rome are analyzed in the framework of a preservation effort of this archaeological area. Thanks to its exceptional size, the Circus hosts many social events with large audience (pop-music, opera concerts, ...
We detected these viruses using PCR/RT-PCR in all samples, which was highly consistent with the viromic results, i.e., viruses with greater abundance in the liver were considerably more prevalent in the liver than in the other organs. Notably, we did not find HEV infection in these ...
Ribosomal RNA was removed using a NEBNext rRNA depletion kit according to the manufacturer’s protocol, followed by amplification using a whole transcriptome amplification WTA2 kit (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany). The sequencing libraries were prepared using a Nextera XT kit (...
WULV was clustered with strawberry associated virus A based on the RdRp nucleotide sequence, but the amino acid sequence did not place it in a defined clade. 3.2.3. “Poty-Like” and “Virga-Like” Viral Sequences Contigs with BLASTx hits of ≤25% significant similarity (E value < 0.001...
For library preparation purposes, the starting material was first converted to DNA. Therefore, the enriched, isolated viral RNA was reverse transcribed to generate cDNA. For the cDNA synthesis and random amplification, a QIASeq FX Single Cell RNA Library Preparation Kit (Qiagen, Hilden, Germany) wa...