The best model was determined with ModelTest-NG v0.1.7 (45), and the tree was built using Phyml v3.3.20220408 (46) using the model best suited for our data set. Visualization was done using iTOL (47). Annotations of densoviruses, Apis mellifera filamentous-like virus, and Apis ...
Furthermore, the year 2022 was compared to 2020, the latter having been characterised by temperatures in line with the last two-decades. The heatwaves do not significantly affect the onset time and the duration of sea breeze, while its cooling effect intensifies during the heatwaves. The urban...
An Illumina double-stranded library was built from 20 mL of extracted DNA for each specimen [26]. We determined the number of indexing cycles using RT-PCR (ThermoFisher, Waltham, MA, USA). Indexing PCRs were set up in 25 mL with a final concentration of 1× Gold Buffer (ThermoFisher,...
was extracted and purified using MiniElute spin columns and Qiagen buffer and was stored at −20 °C. An Illumina double-stranded library was built from 20 mL of extracted DNA for each specimen [26]. We determined the number of indexing cycles using RT-PCR (ThermoFisher, Waltham, MA, ...
and it is found in a clade of other recently discovered fungal-associated and insect-specific virga-like viruses belonging in theVirgaviridaefamily (Figure 3b). On the other hand, the phylogenetic tree built based on the RNA2 polyprotein grouped WaVPV RNA2 with plant viruses from thePotyvirid...