The best model was determined with ModelTest-NG v0.1.7 (45), and the tree was built using Phyml v3.3.20220408 (46) using the model best suited for our data set. Visualization was done using iTOL (47). Annotation
the ISA field was derived from the global inventory byMaucha et al. (2010). In contrast, ICON generates the ISA field using GLC2009 data. Specifically, if an ICON tile belongs to the urban class of the land use dataset GLC2009, its ISA is set to 1; if it falls into any of the re...
Furthermore, the year 2022 was compared to 2020, the latter having been characterised by temperatures in line with the last two-decades. The heatwaves do not significantly affect the onset time and the duration of sea breeze, while its cooling effect intensifies during the heatwaves. The urban...
An Illumina double-stranded library was built from 20 mL of extracted DNA for each specimen [26]. We determined the number of indexing cycles using RT-PCR (ThermoFisher, Waltham, MA, USA). Indexing PCRs were set up in 25 mL with a final concentration of 1× Gold Buffer (ThermoFisher,...
was extracted and purified using MiniElute spin columns and Qiagen buffer and was stored at −20 °C. An Illumina double-stranded library was built from 20 mL of extracted DNA for each specimen [26]. We determined the number of indexing cycles using RT-PCR (ThermoFisher, Waltham, MA, ...
and it is found in a clade of other recently discovered fungal-associated and insect-specific virga-like viruses belonging in theVirgaviridaefamily (Figure 3b). On the other hand, the phylogenetic tree built based on the RNA2 polyprotein grouped WaVPV RNA2 with plant viruses from thePotyvirid...