Proteins in the supernatant were precipitated with protein pellets washed twice with acetone, and the pellet lightly dried under nitrogen. Filter Aided Sample Preparation (FASP) kits were used for protein digestion accord- ing to the manufacturer's instructions. Resultant peptides were snap frozen in...
Nectins are Ca2+-independent immunoglobulin-like cell-cell adhesion molecules which constitute a family of four members. Nectins homophilically and heterophilicallytrans-interact and cause cell-cell adhesion. This nectin-based cell-cell adhesion plays roles in the organization of adherens junctions in ...
31 Cremaster muscles were fixed in periodate/lysine/paraformaldehyde, cryoprotected in 7% sucrose/phosphate-buffered saline, and frozen over liquid nitrogen. Eight-μm sections were prepared on a cryostat, and individual sections stained using RB6-8C5 (anti-Gr-1) to demonstrate neutrophils, FA-11...
For analysis of MPO activity, one lobule of a lung was cut out, immediately frozen in liquid nitrogen, and stored at À 80 °C until use. In the therapeutic protocol, the lethal dose (180 mg kg À 1) and superdose (360 mg kg À 1) of TsV were injected and indomethacin (2 ...
To address this point, in situ hybridization was carried out on paraffin-embedded and frozen brain sections from three nondemented controls and five AD patients. Using specifically designed antisenseAPOEprobes specific in situ hybridization signals were detected glial cells but also in selected neurons ...
Korc and maintained in accordance with ATCC's recommendations. Human Pancreatic Cancer Samples Matched and unmatched frozen surgical samples of normal human pancreatic tissues and pancreatic adenocarcinomas were provided by Dr. A.D. Proia and Dr. D. Tyler (Duke University Medical Center) and ...
Analysis of microbe populations by 16S rRNA gene sequencing Digestate samples (~ 2 g) were taken from the digesters on days 2, 17, 31, 50, 71, 93 and 120 from two replicate digesters under each condition (56 samples in total), snap-frozen in liquid nitrogen and stored at ...
Kids in charge: health and fun play key roles in getting kid-oriented frozen breakfast products into lots of little hands.(frozen food industry ) 来自 findarticles.com 喜欢 0 阅读量: 26 作者: Riell, Howard 年份: 2005 收藏 引用 批量引用 报错 分享 ...
Sonicated α-syn fibrils were aliquoted, snap frozen in liquid nitrogen and stored at − 80 °C. An aliquot of the sonicated fibrils was diluted 4 times in aggregation buffer (50 mM Tris, 150 mM NaCl, pH 7.5) before characterization. Characterization of α-syn fibrils by TEM. The ...
Frozen sections of 55 mm were cut using a microtome to localize the positions of the electrode tips according to the atlas by Paxinos and Watson (1997). Only data from rats having chemitrode tips inside the dPAG were included in the statistical analysis. Immunofluorescence Male Wistar rats ...