acompared to absorption of the wild-type PYP. This implies [translate] afairly well with the absorption within the protein (30), [translate] asubstitution by residues with similar volumes but no [translate] aThe role of the active site residue Glu46 in PYP, which is [translate] ...
P. Madhavapeddi, E.N.G. Marsh, The role of the active site glutamate in the rearrangement of glutamate to 3-methylaspartate catalyzed by adenosylcobalamin- dependent glutamate mutase, Chem. Biol. 8 (2001) 1143-1149.Madhavapeddi P, Marsh ENG. The role of the active site glutamate in the ...
In Escherichia coli serine hydroxymethyltranferase, the hydroxyl group of this conserved tyrosine (Tyr55) is located in a position compatible with a role as hydrogen exchanger in the transaldimination reaction. Because of the location of Tyr55 at the active site, the enhancement of its acidic ...
Water is expected to play an important role since, upon entering the binding site, the ligand has to shed its solvation shell in total or in part, while the water that originally was in the binding site has to rearrange and negotiate its way out of the binding cavity. Not surprisingly, ...
Remarkable structural changes take place during the metal to insulator first-order transition in Pr 0.50 Ca 0.50 CoO 3 : a contraction of the unit-cell volume, the bending of the Co-O-Co bond angle, and a decrease in (Pr,Ca)-O bond distance. However, the Co-O bond length and the ...
Fig. 1. Active site of the WT coproheme-CdChdC (Protein Data Bank: 6XUC) and the variants H118A and A207E obtained by in silico mutations with PyMOL shown in stick representation. Modifiedamino acidsare shown in green, while distal Y135 and proximal H158 are in grey. (For interpretat...
The Molybdenum Cofactor Biosynthetic Protein Cnx1 Complements Molybdate-Repairable Mutants, Transfers Molybdenum to the Metal Binding Pterin, and Is Associ... Molybdenum (Mo) plays an essential role in the active site of all eukaryotic Mo-containing enzymes. In plants, Mo enzymes are important for...
Hydrogen peroxide (H2O2) is recognized to act as a signaling molecule. Peroxiredoxins (Prxs) have the ability to transfer H2O2-derived oxidizing equivalents to redox-regulated target proteins, thus facilitating the transmission of H2O2 signals. It has re
The K201N substitution resides in the Walker A motif, which is believed to be essential for ATPase activity, whereas the E418Q and H421Y mutations are in the Zn2+-binding motif believed to form the active site of the protease domain. As expected, the mutants E418Q and H421Y had no ...
Platelets have long been known for their role in primary haemostasis, but they are now also considered to be components of the immune system and to have a central role in the pathogenesis of IMIDs. In patients with IMIDs, platelets are activated by disease-specific factors, and their ...