A putative sigma factor gene, orf21, was disrupted or overexpressed in the wild-type clavulanic acid (CA) producer Streptomyces clavuligerus NRRL3585 and characterized. An orf21 mutant (Streptomyces clavuligerus HN14) of S. clavuligerus was obtained by insertional inactivation via double-crossover. ...
Accordingly, PrxQ-mediated resistance to adverse stresses mainly relied on the degradation of ROS. The physiological roles of PrxQ in resistance to adverse stresses were corroborated by its induced expression under adverse stresses, regulated directly by the stress-responsive ECF-sigma factor SigH. ...
Spinal inflammatory cytokines (tumour necrosis factor [TNF]-α and interleukin[IL]-1β) expression at day 28 after spinal cord injury (SCI) in wild type (WT) and sigma-1 receptor knockout (KO). (A) Quantification and representative immunoblots of TNF-α and glyceraldehyde 3-phosphate dehydro...
cell signaling hepatocyte growth factor/scatter factor (HGF/SF) lipid signaling Sphingosine kinase (SphK) sphingosine-1-phosphate (S1P) Spns2 trans Introduction Migration of vascular endothelial cells (ECs)2 is a complex process involving protrusion, adhesion, contraction, and retraction, which is impo...
Despite the interest of podxl no much is known about the transcriptional regulation of its gene in different cells. The binding of the Wilms Tumor suppressor factor (WT1) to conserved elements within the Podxl gene promoter results in a potent transcriptional activation [14] that seems to be rel...
Colony-stimulating factor 1 (CSF1) controls the growth and differentiation of macrophages.CSF1R signaling has been implicated in the maintenance of the intestinal stem cell niche and differentiation of Paneth cells, but evidence of expression of CSF1R within the crypt is equivocal. Here we show th...
Previous work established that the principal sigma factor (RpoV) of virulent Mycobacterium bovis, a member of the Mycobacterium tuberculosis complex, resto... AJC Steyn,DM Collins,MK Hondalus,... - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量: 476...
In this in vivo system, all tested arGSTs boosted the titre of the end-product cyanidin-3-O-glucoside (13) by a factor of up to 36.5, while the control GSTs unrelated to anthocyanin biosynthesis were comparable to the control without GST (Fig. 4c,d). To further explore the substrate...
To select residues in coagulation factor XIa (FXIa) potentially important for substrate and inhibitor interactions, we examined the crystal structure of the complex between the catalytic domain of FXIa and the Kunitz protease inhibitor (KPI) domain of a physiologically relevant FXIa inhibitor, ...
Probing the role of sigma πinteraction and energetics in the catalytic efficiency of endo-1,4-β-xylanase. Appl. Environ. Microbiol. 78, 8817–8821 (2012). 21. Johnson, Q. R., Lindsay, R. J., Petridis, L. & Shen, T. Investigation of carbohydrate recognition via computer ...