DNA primaseThe temperature-sensitive yeast DNA primase mutant pri1-M4 fails to execute an early step of DNA replication and exhibits a dominant, allele-specific sensitivity to DNA-damaging agents. pri1-M4 is defective in slowing down the rate of S phase prog...
What is the structure and function of DNA? What is the role of primase in DNA synthesis? What is the role of ligase? What are some of the characteristics of DNA? What are the functions of DNA and through what process does the DNA acquire these functions?
What is the lagging strand in DNA replication? What is the function of DNA polymerase 1? What is the function of DNA polymerase 2? Describe the role Taq polymerase plays in DNA amplification. What does DNA polymerase catalyze? What is the role of primase is the process ...
Surprisingly, even after eliminating both of the identified DNA ligases in yeast, a cdc9-1 dnl4 Delta strain was able to complete DSB repair. Previous studies of asynchronous cultures carrying temperature-sensitive alleles of PCNA, DNA polymerase alpha (Pol alpha), or primase showed that these ...
The 50-kDa primase subunit of Drosophila melanogaster DNA polymerase alpha. Molecular characterization of the gene and functional analysis of the overexpre... The gene encoding the 50-kDa subunit of Drosophila melanogaster DNA polymerase alpha has been cloned. A comparison of the predicted polypeptide...
After primase synthesizes a primer that DNA polymerase α (pol α) can readily elongate, further primase activity is negatively regulated. This occurs within both the context of the four-subunit pol α−primase complex and in the p49−p58 primase complex, indicating that the newly generated ...
Using DNase I footprinting and a gel mobility shift assay, purified DnaA protein was found to bind to four DnaA consensus binding sequences immediately upstream of the five iterons at the RK2 origin of replication. Binding of the TrfA protein to the iterons results in localized strand ...
A DNA Polymerase-α·Primase Cofactor with Homology to Replication Protein A-32 Regulates DNA Replication in Mammalian Cells α-Accessory factor (AAF) stimulates the activity of DNA polymerase-α·primase, the only enzyme known to initiate DNA replication in eukaryotic cells (Gou... Darren,E.,Cas...
Such a model is reminiscent of the role of SSB in the hand-off of RNA primers from primase to the DNA polymerase III holoenzyme during bacterial DNA replication. In this mechanism, primase binds to an RNA primer only when it is simultaneously bound to SSB; the primer/template is handed ...
DNA Replication Repair Recombination and Chromosome Dynamics: An iron-sulfur cluster in the C-terminal domain of the p58 subunit of human DNA primase of the p58 subunit. In the crystal structure of the S. solfataricus primase core, the p48 subunit assembles with the N-terminal half of p58...