Using this method, we have identified a DNA primase activity that copurified with DNA polymerase from the crude protein mixture. The primase catalyzed the synthesis of short RNA primers on single-stranded DNA templates. Among the synthetic templates, the order of preference was poly(dA), poly(...
During replication, the role of DNA primase is to ___. (a) add a nucleotide to the growing DNA chain (b) synthesize a short chain for RNA (c) untwists the double helix at the origin of replication (d) relax the supercoiling of the double helix (e) syn ...
What is the role of topoisomerase in DNA replication? What is the role of DNA methylation in regulating transposons (Transposable Elements, TEs; "jumping genes")? What role does DNA play in phenotypic expression in organisms? What is the role of primase in DNA synthesis? What is th...
The masking activity of the N‐terminus does not require ATP or hexamerization of HpDnaB and can be overcome by deleting the N‐terminus. It can also be neutralized by engaging the N‐terminus in an interaction with a partner like the C‐terminus of DnaG primase. The inhibitory effect of...
Recent reports of tenofovir toxicity in patients taking ART for HIV cannot be explained solely on the basis of off-target inhibition of mitochondrial DNA polymerase gamma (Pol纬). PrimPol was discovered as a primase-polymerase localized to the mitochondria with repriming and translesion synthesis ...
The evolutionary primitive RNA polymerase could do both transcription and priming of DNA replication. Accordingly, abortive cycling would play an important role in evolution at the emergence of DNA world. The priming activity of the primitive RNA polymerase would be taken over by primase later, ...
The masking activity of the N-terminus does not require ATP or hexamerization of HpDnaB and can be overcome by deleting the N-terminus. It can also be neutralized by engaging the N-terminus in an interaction with a partner like the C-terminus of DnaG primase. The inhibitory effect of ...
GINS homologues are found in the and have been shown to interact directly with the and with primase, suggesting a central role for the complex in archaeal replication also. The present review summarizes current knowledge of the structure, function and evolution of the in and , discusses possible...
Primosomes loaded at oriC are composed of only DnaB and DnaG (3), the primase (4), whereas primosomes loaded at recombination intermediates (5) are likely to also include PriA, PriB, PriC, and DnaT (6, 7). The function of these latter four proteins at the replication fork have yet...
This pathway is associated with the core of the DNA replication machinery comprising CDC45, the replicative MCM2-7 hexamer, GINS (altogether forming the CMG complex), primase–polymerase (POLε, -α, and -δ) complex, and additional fork protection factors such as AND-1, CLASPIN (CLSPN), ...