Biochemistry The role of conserved small protein B amino acids in trans-translation STATE UNIVERSITY OF NEW YORK AT STONY BROOK DulebohnDaniel PatrickBacteria possess a unique salvage mechanism for rescuing ribosomes stalled on aberrant mRNAs. This salvage mechanism, termed -translation, is mediated ...
Due to the lack of specificity and sensitivity in the diagnosis of AIH, new biomarkers for diagnosis and monitoring of treatment response are also in urgent need of development. The main site of amino acid metabolism is the liver [18]. According to the nutritional demand, amino acids are ...
HCT116 cells were starved of amino acids in the presence or absence of A769662 (100 µM). d, e A769662 prevents the ability of amino acid starvation or Torin1 to increase ULK1 activity. MEFs were pre-treated with vehicle (−) or A769662 (+) for 30 min, then incubated ...
In an amino acid-rich environment TOR is active and regulates protein translation but also inhibits macroautophagy (hereafter referred to as autophagy). When extracellular amino acids are limiting, autophagy recycles intracellular constituents as a way to provide an alternative source of amino acids (...
Our data identify a molecular function of the C38 methylation introduced by Dnmt2 that can be directly connected to a physiological function, which lies in a system-wide novel post-transcriptional regulation mechanism. Homopolymeric stretches of amino acids other than poly-Asp are widespread in the...
We found that PRAME is significantly enriched with serine (p-value < 0.05), a disorder-promoting amino acid. PRAME was found to have an average disorder score of 16.49% (i.e., moderately disordered) across six per-residue intrinsic disorder predictors. Our IUPred analysis revealed the ...
Arrange the following steps in correct order: (TRANSLATION- ELONGATION): (I) Addition of amino acids takes place one by one in the sequence of codons (II) Ribosome moves from codon to codon along the mRNA in 5'→3' direction. (III) A peptide bond is formed by the enzyme peptidyl trans...
Deletion of trpE led to decreased bacterial attachment and this biofilm defect was restored by exogenous tryptophan or indole. Conclusions Biofilm growth of S. Typhimurium causes distinct changes in gene and protein expression. Our results show that aromatic amino acids make an important contribution ...
Amino acids induce activation of Rag proteins, which mediates translocation of mTORC1 to lysosomal surface. The intra lysosomal amino acids activate mTORC1 in an arginine-dependent manner via interaction of transporter SLC38A9 with the Rag-Ragulator-v-ATPase complex. Cytosolic amino acids engage the...
Peptides with a minimum length of 6 amino acids and up to two missed cleavages were included. Cysteine carbamidomethylation and methionine oxidation were specified as fixed and variable modifications, respectively. The acceptable FDR was set to 1% at the peptide, protein, and modification levels. ...