2023Zhiqiang Huang, Astradeni Efthymiadou, Ning Liang, Rongrong Fan, Eckardt TreuterHuddinge, Sweden) with single-ended output. ChIP-qPCRwas performed with SYBR Green (KAPA Biosystems,07959567001) to validate theChIP-seqresults. CUT&Tag was performed using a published protocol (45). Library sam...
(Thermo Scientific, #89901) containing 1 mM PMSF in Ethanol, 10 μg/ml Aprotinin, 0.4 mM Orthovanadat). After 15 minutes on ice cell lysates were centrifuged 20 minutes at 13000 rpm. The lysates were separated on SDS-PAGE by standard protocol and transferred to nitrocellulose by Western ...