The LightCycler® can be operated using a PC workstation and can utilize a Windows NT operating system. Signals from the samples are obtained as the machine positions the capillaries sequentially over the optical unit. The software can display the fluorescence signals in real-time immediately after...
Amplification and melt analysis were performed using the Roche LightCycler 480 instrument. The reactions were subjected to the following temperature profile: 50° C. for 5 minutes (UNG step), 95° C. for 10 minutes (polymerase activation), followed by 50 cycles of 95° C. for 10 seconds and...
(Cat No 3 246 809, Roche Diagnostics GmbH, Germany) for real-time PCR detection, following the instructions of the manufacturer provided in the kit and using LightCycler Parvo B 19 Standard as the template. The temperature profile as shown in FIG. 6 was set to generate the PCR curve. The...
the still attached and protected peptide was labeled with a 1.5 fold amount of LightCycler-Red 640-N-hydroxy succinimide ester (purchased from Roche Diagnostics GmbH) and triethylamine. After 3 hrs. the resin was washed with Dimethylformamide and Isopropanol until the eluates of the blue resin got...