DNase I digests DNA efficiently Fig.1 Add 1 μg DNA and different activity units of DNaseI in a total reaction volume of 10 μl. Compared with competitor 1 and 2, DNase I of kactus has a stronger digestive effect. Product Description ...
可消化单链或双链DNA,无RNase 市场价:￥400.00 价格: ￥400.00 规格: 1000 U 50,000 U 货号: RE101 RE101-OEM 数量: 立即购买 商品信息 FAQ 说明书下载 产品简介 脱氧核糖核酸酶I(Deoxyribonuclease I),简称DNase I,来源于重组E. coli菌株,是一种可以消化单链或双链DNA的核酸内切酶,不含RNase,可以用于各...
DNase I,RNase-free 脱氧核糖核酸酶 I ,无核糖核酸酶 产品货号: EG22204S 产品规格: 1000 U 产品价格: ￥500 产品介绍 本产品是重组表达来源的DNase I (Deoxyribonuclease I),中文名称为脱氧核糖核酸酶I,是一种可消化单链或双链DNA的脱氧核糖核酸内切酶,它识别并切割磷酸二酯键,产生5'端为磷酸基团,3'端为...
DNase I,不含RNase,规格为1000 Units,浓度为1 U/µL。它是一种核酸内切酶,可消化单链及双链DNA。它可使磷酸二酯键发生水解从而产生含有5'-磷酸基团和3'-OH基团的单脱氧核苷酸和寡脱氧核苷酸。 该款酶活性非常依赖于Ca2+ 水平,可由Mg2+ 或Mn2+ 离子来激活。
本产品是重组表达来源的 DNase l (Deoxyribonuclease I),中文名称为脱氧核糖核酸酶 I,是一种可消化单链或双链 DNA 的脱氧核糖核酸内切酶,它识别并切割磷酸二酯键,产生5' 端为磷酸基团,3'端为羟基的单脱氧核苷酸或单链或双链的寡脱氧核苷酸。 DNase I的活性依赖于 Ca²⁺,并可被二价金属离子 Mg²⁺、...
Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.The enzyme activity is
▪DNase I, RNase-Free (1 U/µL):-20°C ▪10X DNase I Reaction Buffer : -20°C 参考文献: 1. Sambrook, J. et al., (1989) in: Molecular Cloning: A Laboratory Manual (2nd ed.), Cold Spring Harbor Laboratory Press, New York. ...
DNase I (RNase-free) nonspecifically catalyzes the degradation of single- and double-stranded DNA and DNA-RNA hybrids
Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ ...
3.加入1μL 50mM EDTA,65℃孵育10分钟,使DNaseⅠ失活,终止反应。 4.经过纯化得到纯品RNA(纯化方法见附加资料)。 使用说明二:用于RNA试剂盒(吸附柱法)提取过程中的基因组DNA的去除 1.准备DNase I工作液(每个吸附柱需要50μL的工作液)。 工作液配置方法:10μL 10×Reation Buffer(with MgCl2)+2μL DNase...