在一个商业散装RNAseq试剂盒(TruSeq RNA文库制备试剂盒v2)和两个商业单细胞RNAseq试剂盒(NEBNext单细胞/低输入RNA文库制备试剂盒和QIAseq FX单细胞RNA文库试剂盒)中使用SEQURNA证明了高质量的SEQURNA文库(补充图8)。这意味着广泛的合成RNase抑制剂在不同实验条件和RNAseq方法中的适用性。 图3 Sequrna在小体积和...
在一个商业散装RNAseq试剂盒(TruSeq RNA文库制备试剂盒v2)和两个商业单细胞RNAseq试剂盒(NEBNext单细胞/低输入RNA文库制备试剂盒和QIAseq FX单细胞RNA文库试剂盒)中使用SEQURNA证明了高质量的SEQURNA文库(补充图8)。这意味着广泛的合成RNase抑制剂在不同实验条件和RNAseq方法中的适用性。 图3 Sequrna在小体积和...
在一个商业散装RNAseq试剂盒(TruSeq RNA文库制备试剂盒v2)和两个商业单细胞RNAseq试剂盒(NEBNext单细胞/低输入RNA文库制备试剂盒和QIAseq FX单细胞RNA文库试剂盒)中使用SEQURNA证明了高质量的SEQURNA文库(补充图8)。这意味着广泛的合成RNase抑制剂在不同实验条件和RNAseq方法中的适用性。 图3 Sequrna在小体积和...
NEB #名称组分货号储存温度数量浓度 M0303S-20 DNase I (RNase-free)M0303SVIAL-201 x 0.5 ml2,000 units/ml DNase I Reaction BufferB0303SVIAL-201 x 1.5 ml10 X M0303L-20 DNase I (RNase-free)M0303LVIAL-202 x 1.25 ml2,000 units/ml ...
目前,国际上只有少数公司可以生产RNase HII,例如NEB,研究数据表明,Magigen RNase HII产品的性能已经达到国际同类产品的水平。 这款耐高温的RNase HII试剂盒产品来源于人工改造后的嗜热细菌rnhB基因,分子量大小为36kd,C端携带组氨酸标签His tag。 与野生型Tth RNase HII和E.coli RNase HII相比,Magigen RNase HII有更...
(Extended Data Fig.7a,b), which was sufficient to distinguish and assign the RNase R and the 30S subunit densities (Fig.4b). In state II, RNase R has shifted from its location near the mRNA exit channel to the intersubunit surface, where it sits between the head and the body of the...
4 μL of truncated T4 RNA ligase II (NEB; cat. M0373L), 2 μL of RNasin and 20 μL of 50% PEG8000, in 1x R1 buffer. The reaction was left to proceed overnight at 16 °C. The next morning, columns were washed once with 0.5 mL of buffer WB1, once with 0.75...
BRCA1 was incubated for 30 min at 37 °C with 10 nM dsDNA or DNA:RNA hybrid substrate in a reaction buffer containing Tris-HCl pH 7.5, 25 mM; KCl, 90 mM; EDTA pH 8.0, 1 mM; DTT, 1 mM; BSA (NEB), 0.1 mg/ml; RNaseOUT (Invitrogen). The cold competitor ...
Ribosomal RNA was removed by using the NEBNext rRNA depletion kit (New England Biolabs). Strand-specific library preparation was performed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). RNA-seq libraries were quantified with KAPA Library ...
Monarch RNase A 货 号 #T3018LNEB酶试剂 New England Biolabs 发表于2024年2月9日 上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。 产品资料 – 核酸纯化 – 基因组DNA提取 Monarch RNase A 收藏 货号 规格 价格(元) 北京库存 上海库存 广州...