RNase H2 Enzyme—needed for activation of rhPCR primers and PCR product extension Add to Order RNase H2 Enzyme QuantityProductCatalog #Price RNase H2 Enzyme Large - 500 U at 20U/µL 11-03-02-03 $2,275.00 USD RNase H2 Enzyme Small - 50 U at 2U/µL 11-03-02-02 $230.00 USD...
DNA primers containing a single RNA residue and a 3′ blocking moiety (rhPrimers) are activated when cleaved by RNase H2 enzyme. Cleavage occurs on the 5′ side of the RNA base after primer hybridization to the target DNA. Because the primers can only be cleaved after they hybridize to ...
After RNase H2 incision at the rNMPs in DNA the Ribonucleotide Excision Repair (RER) pathway completes the removal, restoring dsDNA. The development of the RNase H2-RED (Ribonucleotide Excision Defective) mutant enzyme, which can process RNA/DNA hybrids but is unable to cleave rNMPs embedded in...
, 1999). Additionally, human RNase H2 has been cloned, but to date the expressed protein has not been shown to be active (Frank et al., 1998). Very recently, we have reported on the effects of several mutations introduced into human RNase H1 on the activity of the enzyme (Wu et al....
7c). Consistently, RNase H2-specific enzyme activity was also reduced in cells derived from patients with WS (Fig. 6c and Supplementary Fig. 7d). Agarose gel electrophoresis under alkaline conditions revealed that ribonucleotides were frequently incorporated into the genomes of cells derived from ...
A. et al. The structure of the human RNase H2 complex defines key interaction interfaces relevant to enzyme function and human disease. J. Biol. Chem. 286, 10530–10539 (2011). Article CAS Google Scholar Sparks, J. L. et al. RNase H2-initiated ribonucleotide excision repair. Mol. Cell...
上海玉博生物科技有限公司做为IDT代理商之产品11-02-12-01-RNase H2 Enzyme Kit 的产品销售公司,专业经营原装进口抗体(单克隆、多克隆)、重组蛋白、检测试剂盒、标准品、细胞因子、胎牛血清、细胞培养类等生物试剂以及耗材。 ATCC sigma anatrace caroteNature NIBSC medkoo thermo life CIL macrocyclics invitrogen...
The Structure of the Mammalian RNase H2 Complex Provides Insight into RNA·DNA Hybrid Processing to Prevent Immune Dysfunction crystal structure of the three-protein mouse RNase H2 enzyme complex to better understand the molecular basis of RNase H2 dysfunction in human autoimmunity... NM Shaban,S Ha...
Recombinant Platelet Activating Factor Acetylhydrolase 2 (PAFAH2)产品名称:血小板激活因子乙酰水解酶2(PAFAH2)重组蛋白物种:Mouse,小鼠SD-PLA2; Serine-dependent phospholipase A2; Platelet-activating factor acetylhydrolase 2, cylasmic Eukaryotic Angiotensin I Converting Enzyme 2 (ACE2)产品名称:血管紧张素转化...
Eukaryotic RNase H2 has evolved markedly from its prokaryotic ancestry. In both archaea and bacteria, the RNase H2 enzyme consists of a single protein, while eukaryotic RNase H2 is a complex containing three protein subunits, all of which are required for activity (5). The RNASEH2A subunit exh...