RNase H1 has become an essential tool to uncover the physiological and pathological roles of R-loops, three-stranded structures consisting of and RNA鈥揇NA hybrid opposite to a single DNA strand (ssDNA). RNase H1 degrades the RNA portion of the R-loops returning the two DNA strands to ...
1.1 RNase H2 RNase H普遍存在于各种生物中,主要分为2个家族,目前根据其氨基酸序列的不同分为RNase H1型和RNase H2型。RNase H1包括细菌RNase HⅠ、哺乳动物RNase HⅡ、逆转录酶的RNase H域和古细菌RNase HⅠ,RNase H2包括细菌RNase HⅡ、细...
Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic ...
et al. Specific recognition of RNA/DNA hybrid and enhancement of human RNase H1 activity by HBD. EMBO J. 27, 1172–1181 (2008). Article CAS PubMed PubMed Central Google Scholar Nguyen, H. D. et al. Functions of replication protein A as a sensor of R loops and a regulator of ...
[18] IF8.579 circPTCH1 promotes invasion and metastasis in renal cell carcinoma via regulating miR-485-5p/MMP14 axis. Theranostics[19] IF8.469 Circular RNA hsa_circ_0043280 inhibits cervical cancer tumor growth and metastasis via miR-203a-3p/PAQR3 axis. Cell Death & Disease...
Both RNase H1 and RNase H2 complexes resolve R-loop structures, consisting of double-stranded RNA-DNA hybrid and non-template single-stranded DNA, which are formed during transcription19,20. Unlike RNase H1, which can only process R-loops, RNase H2 can recognise ribonucleoside monophosphates (...
coli RNase H1 (Lima and Crooke, 1997). Any structure in the duplex substrate was found to have a significant negative effect on the cleavage rate. Further, cleavage of selected sites was inhibited entirely, and this was explained by steric hindrance imposed by the RNA loop traversing either ...
RNase R是一种来源于大肠杆菌RNR超家族的3’-5’核糖核酸外切酶,可从3’-5’方向将RNA逐步切割成二核苷酸和三核苷酸。RNase R可消化几乎所有的线性RNA分子,但不易消化环形RNA、套索结构或3’突出末端少于7个核苷酸的双链RNA分子。RNase R是circRNA的鉴定和富集实验必备工具酶,可消化线性RNA以使环形RNA(circRNAs)...
α-hydroxytropolones (αHTs) are a class of anti-RNase H inhibitors that can inhibit the HIV, hepatitis B virus, and human RNases H1; however, it is unclear how these inhibitors could be developed to distinguish between these enzymes. To accelerate the development of selective RNase H ...
近日,广州市妇女儿童医疗中心优生围产研究所李卫、刘超团队联合上海科技大学孙博团队在Nucleic Acids Research杂志在线发表了题为“RNase H1 facilitates recombinase recruitment by degrading DNA–RNA hybrids during meiosis”的研究论文,揭示了R-loop累积导致无精症的作用机制。