在控制R-loop动态变化的因子中,进化保守的RNase H1可以特异性消化RNA:DNA杂合链中的RNA,从而直接从基因组中去除R-loop,维持基因组的稳定性。孙前文实验室前期工作在拟南芥中鉴定到三个RNase H1类的R-loop移除酶AtRNH1A、AtRNH1B和AtRNH1C(The Plant Cell 2017),并详细解析了叶绿体定位的AtRNH1C在维持质体基因组...
在这些因素中,进化保存的RNASE H蛋白(H1和H2)专门消化RNA:DNA杂交中的RNA莫伊蒂,从而直接去除基因组中的R循环[7]。哺乳动物 RNase H1 与细胞核和线粒体双局部化。RNase H1功能丧失导致人类严重疾病,小鼠的RNase H1淘汰突变体是胚胎致命[8,9]。RNase H1 对于哺乳动物线粒体 DNA (mtDNA) 复制([10]; [11]...
1.1 RNase H2 RNase H普遍存在于各种生物中,主要分为2个家族,目前根据其氨基酸序列的不同分为RNase H1型和RNase H2型。RNase H1包括细菌RNase HⅠ、哺乳动物RNase HⅡ、逆转录酶的RNase H域和古细菌RNase HⅠ,RNase H2包括细菌RNase HⅡ、细...
R-loopRNase H1RNase H2In Saccharomyces cerevisiae, genome stability depends on RNases H1 and H2, which remove ribonucleotides from DNA and eliminate RNA–DNA hybrids (R‐loops). In Schizosaccharomyces pombe, RNase H enzymes were reported to process RNA–DNA hybrids produced at a double‐strand ...
Human RNase H1 was shown to bind selectively to “A-form” duplexes with approximately 10- to 20-fold greater affinity than that observed for E. coli RNase H1.19,20 Finally, human RNase H1 displays a strong positional preference for cleavage, i.e., the enzyme cleaves between 8 and 12 ...
et al. Specific recognition of RNA/DNA hybrid and enhancement of human RNase H1 activity by HBD. EMBO J. 27, 1172–1181 (2008). Article CAS PubMed PubMed Central Google Scholar Nguyen, H. D. et al. Functions of replication protein A as a sensor of R loops and a regulator of ...
Mutations in genes such as MED5, MED12, STB3, SIN3, RRP6, TRF4, or PAF1 cause a significant increase in YAC loss that is suppressed by RNase H1 overexpression (Wahba et al., 2011). The impact of some of the mutations over different DNA regions varies. Show abstract R loops: New ...
RNase H1 restricts telomeric hybrids and C-rich ssDNA in ALT cells In search of cellular regulators of telomeric hybrids, we first transfected U2OS and HeLa cells with short interfering RNAs (siRNAs) against the shelterin components TRF1 and TRF2. Protein depletions were very efficient, generated ...
[18]IF8.579circPTCH1 promotes invasion and metastasis in renal cell carcinoma via regulating miR-485-5p/MMP14 axis.Theranostics [19]IF8.469Circular RNA hsa_circ_0043280 inhibits cervical cancer tumor growth and metastasis via miR-203a-3p/PAQR3 axis.Cell Death & Disease ...
Furthermore, either inhibition of the RTase activity or depletion of endogenous RNase H1 results in the promotion of virus proliferation. Altogether, our results provide intriguing insights into the antiviral mechanism of mESCs and the antiviral function of endogenized retroviruses and cellular RNase ...