An RNA-directed DNA polymerase was isolated from the peripheral blood leukocytes of a patient with acute myelomonocytic leukemia by successive purification... RE Gallagher,GJ Todaro,RG Smith,... - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量: 80发表...
We find RNA:DNA hybrids to occupy millions of base pairs in the human genome. A directional sequencing approach shows the RNA component of the RNA:DNA hybrid to be purine-rich, indicating a thermodynamic contribution to their in vivo stability. The RNA:DNA hybrids are enriched at loci with d...
Abt. Originale C: Allgemne, angewandte und kologische MikrobiologieStetter, K. O., J. Winter, and R. Hartlieb. 1980. DNA-depen- dent RNA polymerase of the archaebacterium Methanobacte- rium thermoautotrophicum. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe ...
The combination of this engineered DNA polymerase with qPCR has achieved expeditious quantification of Nm88. In addition to the engineered thermostable KlenTaq DNA polymerase variant, two other DNA polymerases, Tth and Bst, also have reverse transcriptase activity and exhibit distinct capacities to ...
The synthesis of ribosomal RNA (rRNA) precursor molecules by RNA polymerase I (Poll) terminates with the dissociation of the protein-DNA-RNA ternary complex. Based on in vitro results the mechanism of Pol I termination appeared initially to be rather conserved and simple until this process was ...
Elongin is a heterotrimeric elongation factor for RNA polymerase (Pol) II transcription that is conserved among metazoa. Here, we report three cryo-EM structures of human Elongin bound to transcribing Pol II. The structures show that Elongin subunit ELOA
DNA聚合酶(DNA polymease)DNA聚合酶原核生物DNA聚合酶IDNA聚合酶nDNA聚合酶m催化活性53,35,5,f3z聚合 核酸外切核酸外切+功能切去引物RNA,补上正确的DNA片段与修复有关(活性低)负责链的延长(活性高)TABLE 25-1 Comparison of DNA Polymerases of E. coliDNA polymeraseIIIIIStructural gene*polApolBpol 5、C ...
Here we provide the first example of an archaeo-viral transcription factor that directly targets the host RNA polymerase (RNAP) and efficiently represses its activity. ORF145 from the temperate Acidianus two-tailed virus (ATV) forms a high-affinity complex with RNAP by binding inside the DNA...
DNA-binding domain DE: Differentially expressed DES: Desmin DUSP1: Dual specificity phosphatase 1 ELL2: Elongation factor 2 RNA polymerase II EMBL: The European Molecular Biology Laboratory FABP4: Fatty acid binding protein 4 DKK2: Dickkopf WNT signaling pathway inhibitor 2 FDR: False...
Lankenau D-H (1999) DNA-Reparatur und Transposons in der Drosophila Keimbahn (Habilitation). In: DKFZ (ed) Universität Heidelberg, Ladenburg Google Scholar Lankenau D-H (2006) Germline double-strand break repair and gene targeting in Drosophila: a trajectory system throughout evolution. In:...