replication- DNA is used as a template to make more DNA, resulting in 2 DNA molecules each with one old and one new strand. In transcription DNA is used as a template to make RNA (mRNA). In translation the sequence of mRNA is decoded into a protein...
Tracking transcription–translation coupling in real time Single-molecule fluorescence microscopy experiments with a recombinantEscherichia colitranscription–translation system provides insights into the coordination of the transcription and translation machineries mediated by the intervening mRNA. ...
Pervasive transcription generates numerous unadenylated RNAs, usually degraded by the NEXT/nuclear exosome pathway. Here, the authors show that, upon NEXT inactivation, these RNAs are removed by compensatory RNA decay pathways relying on RNA 3′end A- or U-tailing. ...
We measured the transcription and splicing dynamics in real time using high-throughput imaging and analysis. The fluorescent intensity at transcription site (TS) was tracked in single cells over 12 h with 100-s intervals (Figure 1B; Video S1). The genes showed discrete ON and OFF periods dire...
Despite the overall lack of structures near start and stop codons, we were able to detect structures that directly block the start and stop codons in a subset of mRNAs, suggesting that some of them may have consequences on mRNA translation. Here shows an example of conserved structure covering...
The importance of tandem repeats was reported in studies on the transcription, translation, and regulation of promoter activities [59,60]. Using the MISA web service and Linux version of the Tandem Repeats Finder (v4.09), we identified 78 SSRs and 9 long tandem repeats (Table S4). Among ...
Reverse transcription reactions used 4 ng of RNA from adipose tissue or 8 μL of an aqueous solution of RNA from plasma. Amplification and registration of the fluorescent signal was performed in a 7500 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) using the qPCRmix-HS ...
单细胞标签逆转录测序技术(single-cell-tagged reverse transcription sequencing, STRT-seq)能够在单细胞水平上实现TSS的回贴(mapping)。STRT-seq技术使用生物素化的模板转换oligos来生成cDNA,被磁珠捕获后,就在5ʹ末端进行片段化,产生短的cDNA标签。作为CAGE基础的5ʹ末端的加帽技术是由日本理化所(Riken)开发的,...
RNA Transcription To build an RNA molecule, the area around a DNA gene must first relax and the two strands must temporarily separate. The separation allows an enzyme complex containing RNA polymerase to fit into a space and attach to the gene's starting area, or promoter, on one of the ...
The processes of transcription, RNA processing (in eukaryotes), and translation constitute the pathway that leads to the conversion of genetic information in the linear sequence of bases in genomic DNA into the linear amino acid sequences of functional proteins. Thus, DNA undergoes transcription to ...