The Inside Story: DNA to RNA to ProteinHortin, Glen LAmericanassociationforclinicalchemistry IncWitkowski, J (2005) The Inside Story – DNA to RNA to Protein. Cold Spring Harbor Press, New York
1. Ramanathan M, Porter DF, Khavari PA, et al. Methods to study RNA-protein interactions. Nat Methods. 2019 Mar;16(3):225-234. doi: 10.1038/s41592-019-0330-1.
蛋白质进出细胞核主要依靠核定位序列或者核输出序列，与核转运蛋白结合，从而形成转运复合物。而转运复合物与NPC结合后能够改变NPC的构象，使之从胞质面转运至核质面。而RNA被转运出细胞核也是一种主动运输的过程——研究表明，真核生物的RNA，都是与相关蛋白结合在一起，形成各种核糖核蛋白颗粒，其含有核定位序列，...
Protein–RNA interactions have critical roles in all aspects of gene expression. However, applying biochemical methods to understand such interactions in living tissues has been challenging. Here we develop a genome-wide means of mapping protein–RNA binding sites in vivo, by high-throughput sequencing...
基于免疫沉淀(Immunoprecipitation,IP)的技术是我们研究蛋白与其它大分子类分子互作的关键技术之一,比如研究蛋白与蛋白互作的Co-IP,蛋白与RNA互作的RIP,蛋白与DNA互作的ChIP,其基本原理就是通过要研究目的蛋白的抗体进行免疫共沉淀,接下来根据要检测的分子类型通过各种方法进行检测。
3.Silencing of the mRNA-binding protein HuR increases the sensitivity of colorectal cancer cells to ionizing radiation through upregulation of caspase-2. Cancer Lett 393:103-112 (2017). 4.Genomic maps of long noncoding RNA occupancy reveal principles of RNA-chromatin interactions. Mol Cell 44(4...
【摘要】近年来,针对肝病有了很多新的治疗策略,包括基于mRNA递送和RNAi技术的RNA疗法及靶向DNA的基因编辑技术等。其中,将mRNA分子递送至靶细胞中可以有效恢复缺失或无功能活性的蛋白表达,而小干扰RNA及反义寡核苷酸等则可靶向沉默致病基因或...
Topoisomerase 3β (Top3β) can associate with the mediator protein Tudor domain-containing protein 3 (TDRD3) to participate in two gene expression processes of transcription and translation. Despite the apparent importance of TDRD3 in binding with Top3β and directing it to cellular compartments cr...
Approaches to footprint RNA-protein binding sites 理解RBP生物学功能和分子机制的关键是鉴定所有RNA转录本的集合。此外,RBP的RNA结合位点或“足迹”(footprint)也可以帮助我们进一步了解其生物学活性。因此早期的研究主要依靠体外选择,利用RBP对固定...
A protein condensate formed by multivalent interactions between the long non-coding RNA Xist and specific RNA-binding proteins drives the compartmentalization required to perpetuate gene silencing on the inactive X chromosome. Amy Pandya-Jones ,Yolanda Markaki ...