2024年5月13日,中山大学宋尔卫团队在Cell Reports Medicine在线发表题为“Advancements in clinical RNA therapeutics: Present developments and prospective outlooks”的综述论文,该文综述了临床RNA治疗目前的发展和前景展望。 该综述探讨了RNA在疾病中的意义,并按时间顺序概述了RNA疗法的发展。此外,总结了RNA筛选设计的...
an abundant cellular RNA that serves as a negative control. Majority of TERC RNAs (~88%) present in the cell were pulled down by performing ChIRP, whereas only
the research on previously uncharacterized longer noncoding RNAs gradually becomes a frontier in the field. At present, large amounts of intermediate-sized and long noncoding RNAs have been identified in model plants including Arabidopsis, rice, maize, and Medicago...
2. Sarah Geisler & Jeff Coller. RNA in unexpected places: long non-coding RNA functions in diverse cellular contexts. Nature Reviews Molecular Cell Biology 2013, 14: 699–712. doi:10.1038/nrm3679 3. Laura M. Beaver, Rachael Kuintzle, Alex Buchanan et al. Long ...
min.cell = 3, min.features = 200) pbmc.obj # An object of class Seurat # 13714 features across 2700 samples within 1 assay # Active assay: RNA (13714 features, 0 variable features) # 1 layer present: counts # 细胞名称重命名 pbmc.obj <- RenameCells(pbmc.obj, add.cell.id = "PBMC...
In conclusion, scRNA-seq technology allows analysis of the single-cell transcriptome with high sensitivity, high precision and high throughput. In addition, the further development of scRNA-seq combined with multiomics will enable obtaining a more comprehensive understanding of cell types and cell state...
Of the many types of RNA, the three most well-known and most commonly studied aremessenger RNA(mRNA),transfer RNA(tRNA), andribosomal RNA(rRNA), which are present in all organisms. These and other types of RNAs primarily carry out biochemical reactions, similar to enzymes. Some, however, ...
cell sorting (FACS). RNA was extracted from each coacervate and mRNA was converted to cDNA and sequenced upon library preparation. RNAs present in each sequenced coacervate were computationally identified and quantified.bSchematic illustration of cross comparisons of several parameters (RNA length, ...
we tested whether maxRNAs are present in primary human cells under physiological conditions and whether their presence is cell-type specific. We chose primary PBMCs for these tests, considering their heterogeneity and frequent interactions among each other and with other cell types. We collected 120...
而科学界对于其精确性、安全性和伦理性的持续探讨,也为CRISPR技术的健康发展提供了重要的指导。2月29日Cell杂志也对此发表了一篇综述“Past, present, and future of CRISPR genome editing technologies”。 撰文| K CRISPR基因编辑的过去 CRISPR技术的起源与发展...