In light of the emergence of rifamycin-resistant bacteria, development of new RNA polymerase inhibitors that kill rifamycin-resistant bacteria with high bioavailability is urgent. Structural analysis of bacterial RNA polymerase in complex with inhibitors by crystallography and cryo-EM indicates that RNA ...
Gene expression requires synthesis of a variety of RNAs including protein-coding messenger RNAs (mRNAs), ribosomal RNAs (rRNAs), and transfer RNAs (tRNAs). RNA synthesis is carried out by a protein enzyme called RNA polymerase (RNAP) that proceeds with a unique enzymatic reaction making various...
RNA polymerase (RNAP or RNApol) is an enzyme that producesRNA. In cells, RNAP is needed for constructing RNA chains fromDNAgenes as templates, a process called transcription. RNA polymerase enzymes are essential to life and are found in all organisms and many viruses. In chemical terms, RNA...
3.8.1RNA polymerase RNA polymerase(DNA-directed RNA polymerase) is an enzyme that synthesizes RNA from a DNA template. The synthesis of RNA requires DNA as a template, ensuring that the base composition of the RNA faithfully reflects that of the DNA. In addition, as in DNA synthesis, all ...
美国耶鲁大学Eduardo A. Groisman研究组在Science发文题为Bacteria require phase separation for fitness in the mammalian gut,发现多形拟杆菌在小鼠肠道中的适应性依赖于转录终止因子Rho的相分离能力。 图片 相分离指的是蛋白质-蛋白质或者蛋白质-RNA之间的依靠弱相互作用形成生物聚集体,这些聚集体形成非膜包被的组分...
1. Chen, J., et al. (2021). Diverse and unified mechanisms of transcription initiation in bacteria.Nat. Rev. Microbiol.19, 95–109. 2. Campbell, E.A., et al. (2001). Structural mechanism for rifampicin inhibition...
Rifamycins, a group of bacterial RNA polymerase inhibitors, are the first-line antimicrobial drugs to treat tuberculosis. In light of the emergence of rifamycin-resistant bacteria, development of new RNA polymerase inhibitors that kill rifamycin-resistant bacteria with high bioavailability is urgent. Str...
Finally, all neomurans use several kinds of RNA polymerase, whereas bacteria use only one. 另外,所有新壁總域成員具有好幾種RNA聚合酶,而真細菌只有一種。 LASER-wikipedia2 RNA polymerase II (RNAP II and Pol II) is a multiprotein complex. RNA聚合酶II(英语:RNA polymerase II,亦被称为RNA...
RNA polymerase (RNAP) in a loci-dependent manner. Further analysis shows that the stalling is caused by the disruption of transcription-translation coupling. Anti-intuitively, the stalled RNAPs subsequently induce lesions to the DNA via transcription-coupled repair. While most of the bacteria are ...
HelD during transcription complex recycling (Fig.1b, c, Table1; Supplementary Fig.2, Movie1). RNAP in theFirmicutesis the smallest multi-subunit polymerase17,18, and given the industrial and clinical importance of this group of bacteria this complex will serve as an invaluable reference ...