OBJECTIVE:Prior studies have demonstrated an increased risk of cancer-associated scleroderma in patients with anti-RNA polymerase III (anti-RNAP III) autoantibodies as well as in patients who are triple-negative for anticentromere (anti-CENP), anti-topoisomerase I (anti-topo I), and anti-RNAP ...
随着对单股正 encephalomyocarditisvirusRNA polymerase in hte 链 RNA病毒31UTR的深人研究,3vUTR在病毒基 3l_noncodingregionoftheviralRNA [J],Nucleic 因组复制、转录、翻译 中的功能将会逐步被揭示, AcidsRes,1995,23(3):377-382. 为基因药物的设计提供更有力的理论支持。 [12]DengR,BrockKV.5Tnad3’unr...
RNA polymerase III (Pol III) includes two alternate isoforms, defined by mutually exclusive incorporation of subunit POLR3G (RPC7α) or POLR3GL (RPC7β), in mammals. The contributions of POLR3G and POLR3GL to transcription potential has remained poorly d
a highly expressed BLV micro-RNA cluster has been reported, suggesting that the BLV silencing is not complete. Here, we demonstrate thein vivorecruitment of RNA polymerase III to the BLV miRNA cluster both in BLV-latently infected cell lines and...
poly(A)在细胞质中会被更新。RNase tear it down, and poly(A) polymerase builds it back up. 但总的趋势是poly(A)不断缩短,poly(A)耗尽时,mRNA也就面临降解。 3 mRNA加工事件的协调 3.1 Rpb1的CTD介导加工过程 加帽、剪接和多腺苷酸化的酶都与...
根据本发明,术语“分离的核酸”意指核酸(i)例如通过聚合酶链反应(polymerasechainreaction,pcr)在体外进行扩增,(ii)通过克隆重组产生,(iii)例如通过切割和通过凝胶电泳进行分离而被纯化,或(iv)例如通过化学合成来合成。核酸可用于引入细胞中,即细胞的转染,特别地以可通过从dna模板体外转录来制备的rna的形式。此外,可...
3.3.1RNA-dependent RNA polymerase The replication of SARS-CoV-2 is dominated by a replication/transcription complex that contains several subunits. The complex is composed ofviral nonstructural proteins(nsp), and the core of the complex is the RNA-dependentRNApolymerase (RdRp) in nsp12. The RdRp...
Ribonucleic acid polymerase III (RNAP III), the most complex of the three nuclear polymerases present in eukaryotic cells, contains 17 subunits and synthetizes various small RNA components. The most common autoantibody response in patients with systemic sclerosis (SSc) is referred to recog...
Specifically in the liver, mature miR-122 is 3′ adenylated by PAP associated domain containing 4 (PAPD4), a poly(A) polymerase, which increased stability51. 特别是在肝脏中,成熟的 miR-122 被含有 4 (PAPD4) 的 PAP 相关结构域 3' ...
according to Watson-Crick base-pairing interactions. Supercoiling plays an important part in polymerase activity because of the unwinding and rewinding of DNA. Because regions of DNA in front of RNAP are unwound, there is compensatory positive supercoils. Regions behind RNAP are rewound and negativ...