RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis Emily G. Kaye, Kavyashree Basavaraju, Geoffrey M. Nelson, Helena D. Zomer, Debarun Roy, Irene Infancy Joseph, Reza Rajabi-Toustani, Huanyu Qiao, Karen Adelman & ...
Antibody raised against purified RNA polymerase II reacted with several enzyme subunits in "Western" blots of purified polymerase and impure enzyme fractions. Immunofluorescence staining was used to visualize RNA polymerase II in the nuclei of a nematode squash preparation and the nucleoplasm of ...
Furthermore, immunofluorescence analysis revealed that Cet-TPL was effectively internalized and transported into lysosomes of EGFR-overexpressing cells. Cet-TPL effectively led to degradation of RNA polymerase II (Pol II) and demethylation of histone H3 lysines, and significantly induced apoptosis in ...
RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence ...
the complex was lysed at 4 °C and randomly fragmented into 500–1000 bp fragments by means of ultrasonification. The obtained supernatant was collected and divided into three tubes. The positive-control antibody RNA polymerase II, negative-control antibody normal rabbit IgG, and rabbit anti-...
(> 0.6), such as 60 S ribosomal subunits, mitochondrial complexes II and IV, RNA polymerase I and III, RNA exosome and mediator complex (Supplementary Fig.4and Supplementary Data5). We also analyzed the spliceosomal B complex, which contains 46 proteins from the U2, U4-U6, U5 complexes...
蛋白别名:3'-5' exoribonuclease; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; EC 2.7.7.6; MGC75453; Pol II S2p;...
Howe JG, Shu MD: Epstein-Barr virus small RNA (EBER) genes: unique transcription units that combine RNA polymerase II and III promoter elements. Cell. 1989, 57: 825-834. 10.1016/0092-8674(89)90797-6 Article CAS PubMed Google Scholar Niller HH, Salamon D, Ilg K, Koroknai A, Banati ...
Our data suggest that topoisomerase IIα is a component of the initiation-competent RNA polymerase Iβ complex and interacts directly with RNA polymerase I-associated transcription factor RRN3, which targets the polymerase to promoter-bound SL1 in pre-initiation complex formation. In cells, activation...
2A). To examine the subcellular localization of endogenous EVA1A, we stained HepG2 cells with anti EVA1A antibody before and after depletion of EVA1A-AS. Endogenous EVA1A is located mainly at the perinuclear region in untreated cells, but upon depletion of EVA1A-AS, EVA1A is distributed ...