RNA PolymeraseCellular CompartmentProduct of Transcriptionα-Amanitin Sensitivity I Nucleolus All rRNAs except 5S rRNA Insensitive II Nucleus All protein-coding nuclear pre-mRNAs Extremely sensitive III Nucleus 5S rRNA, tRNAs, and small nuclear RNAs Moderately sensitiveRNA...
Eukaryotic RNA polymerase I (Pol I) transcribes ribosomal DNA and generates RNA for ribosome synthesis. Pol I accounts for the majority of cellular transcription activity and dysregulation of Pol I transcription leads to cancers and ribosomopathies. Desp
2024年4月19日,武汉大学肖锐团队在Genome Biology在线发表题为“RBM22 regulates RNA polymerase II 5′ pausing, elongation rate, and termination by coordinating 7SK-P-TEFb complex and SPT5”的研究论文,该研究描述了剪接因子RBM22...
not those "seen" by other sigma factors. This simple example illustrates how transcription can be regulated in both cis and trans to cause changes in cell function. Therefore, while bacteria accomplish transcription of all genes using a single kind of RNA polymerase, the use of different sigma ...
RNA polymerase II Transcription Mukesh KumarVenkat Ramani, ...YanZhang, inJournal of Molecular Biology, 2021 Introduction RNA polymerase II(Pol II) is a multi-subunit enzyme involved in transcribing all protein-coding genes in eukaryotes.1,2The largest subunit of Pol II (RPB1) contains a series...
Many developmental genes exhibit promoter-proximal paused RNA polymerase II (Pol II) ~30–60 bp downstream of the TSS [22]. To measure pausing, we calculated the pausing index from the ratio of PRO-seq reads mapping to the promoter (from 50 bp upstream of the TSS to 100 bp downstream of...
Assembled RNA polymerase III (Pol III) complexes exert local effects on chromatin processes, including influencing transcription of neighboring RNA polymerase II (Pol II) transcribed genes. These properties have been designated as `extra-transcriptional'
RNA干扰系列的第一篇。 前言: 很多年以前,分子生物学家就开始利用反义RNA抑制活细胞内特定基因的表达。最初的基本思路是与mRNA 互补的反义RNA可与mRNA互补配对,进而抑制其翻译。这种策略通常可以发挥作用,但这种逻辑推理不完善。 此外,从1990年开始,分子生物学家就注意到在不同的转基因生物体内,有时会表现与期望相...
Equal amounts (2 μg) of RNA was diluted in 7.5 μL of autoclaved ddH2O for each sample (n = 4 biological replicates of BAT sampled from control and torpid ground squirrels) before adding 1.0 μL of 10× Poly-A polymerase buffer, 1.0 μL of 1 mM ATP and 0.5 Units of E. coli ...
The most utilized small RNA pathway in plants appears to be the one involved in silencing repeated sequences including retroelements and transposons, and is dependent on the activities of RNA-dependent RNA polymerase (RDR2) and the Dicer-like protein (DCL3) to produce a large number of a hig...