Learn about the process of transcription inside a cell with a focus on the structure and function of the RNA polymerase ii in the process.
RNA聚合酶Ⅱ(英语:RNA polymerase II,亦被称为RNAPⅡ或PolⅡ)是一个存在于真核生物细胞中的酶。它催化脱氧核糖核酸转录,合成mRNA及大多数snRNA和微RNA的前体[2][3]。RNA聚合酶Ⅱ是含12个亚基的550kDa大的复合物,也是RNA聚合酶中被研究得最多的一类。在它结合到基因上游的启动子并开始转录前需要大量转录因子...
Elongin is a heterotrimeric elongation factor for RNA polymerase (Pol) II transcription that is conserved among metazoa. Here, we report three cryo-EM structures of human Elongin bound to transcribing Pol II. The structures show that Elongin subunit ELOA
During transcription-coupled DNA repair (TCR), RNA polymerase II (Pol II) transitions from a transcriptionally active state to an arrested state that allows for removal of DNA lesions. This transition requires site-specific ubiquitylation of Pol II by the CRL4CSA ubiquitin ligase, a process that...
与这两项工作同一时期,2018年12月21日,德国马普研究所的Patrick Cramer研究组在Nature Communications上发表了题为Structure of transcribing RNA polymerase II-nucleosome complex的工作【11】。这篇文章获得和解析了RNA聚合酶II暂停在核小体SHL(-5)处的电镜结构,但是它只有这一个结构快照,提供的动态机制信息相对少很...
Figure 1.1.The structure of eukaryotic promoters. Eukaryotic gene promoters are composed of discrete binding sites for multiple transcription factors dispersed over long distances (usually several thousands of base pairs). General transcription factors (TFIIX) for RNA polymerase II (Pol II) interact wit...
与这两项工作同一时期,2018年12月21日,德国马普研究所的Patrick Cramer研究组在Nature Communications上发表了题为Structure of transcribing RNA polymerase II-nucleosome complex的工作【11】。这篇文章获得和解析了RNA聚合酶II暂停在核小体SHL(-5)处的电镜结构,但是它只有这一个结构快照,提供的动态机制信息相对少很...
基因表达的调控在所有生命王国中都是至关重要的过程。自从发现核糖体(ribosome)和RNA聚合酶(RNA polymerase)等分子机器以来,研究人员逐渐认识到RNA在基因调控中的核心作用。特别是一些古老的RNA调控机制,如核酶(ribozymes)和RNA结构开关,展示...
RNA polymerase II/TFIIF structure and conserved organization of the initiation complex. The structure of an RNA polymerase II/general transcription factor TFIIF complex was determined by cryo-electron microscopy and single particle analysis. D......
During transcription, RNA polymerase II (Pol II) associates with the conserved elongation factor DSIF. DSIF renders the elongation complex stable and functions during Pol II pausing and RNA processing. We combined cryo-EM and X-ray crystallography to determine the structure of the mammalian Pol II...