除非另有说明,本发明的实施将采用分子生物学(包括重组技术)、微生物学、细胞生物学和生物化学的常规技术,这落入本领域技术范围内。这样的技术在文献中有充分的说明,例如:《分子克隆实验指南》,第2版,Sambrook等人,1989;Oligonucleotide Synthesis,MJ Gait编辑,1984;Animal Cell Culture,R.I.Freshney编辑,1987;Methods...
MerMade 48X (1) Product Type MerMade 4 Column Positions (2020 Model) Item Id: AXMM4 The MerMade oligonucleotide synthesizer is designed to synthesize DNA, RNA and LNA oligonucleotides in column format using standard or...
be an effective therapeutic strategy, especially considering recent FDA approval of antisense oligonucleotide-based therapies for the treatment of neurodegenerative disorders [22]. With the goal of identifying a novel oncogenic lncRNA that could be targeted with antisense oligonucleotides to treat TNBCs an...
Oligonucleotide sequence mapping of large therapeutic mRNAs via parallel ribonuclease digestions and LC-MS/MS. Anal. Chem. 91, 8500–8506 (2019). Article CAS PubMed Google Scholar Riddle, D. S. et al. Functional rapidly folding proteins from simplified amino acid sequences. Nat. Struct. Biol...
templates be completely double stranded. Partially double-stranded oligonucleotide templates with a double-stranded T7 promoter sequence may be used in the T7 Quick High Yield RNA Synthesis Kit, but yields may be variable and in general are lower than when fully double stranded templates are used...
The present invention relates to a novel oligonucleotide primer used for the synthesis of 5'-capped RNA. The novel oligonucleotide primer for RNA capping provided as chemical formula 1 in the present invention can be usefully utilized in the fields of nucleic acid therapeutic agents or vaccines....
DIG Nucleic acid Detection Kit 地高辛核酸检测试剂盒 11175041910 Roche Kit 5165 NBT/BCIP显色法检测, 灵敏度可达0.1pg同源DNA; 可检测40张100cm2杂交膜。-20℃保存。 DIG Oligonucleotide 3’-End Labeling Kit 寡核苷酸3’末端地高辛标记试剂盒 3353575910 ...
The MICROBExpress kit from Ambion (Austin, TX) is the most widely used commercial kit in depleting rRNA from bacteria. One end of the captureoligonucleotideprobes targets conserved regions of 16S and23S rRNAs, and the other end contains supplementary sequence to a secondary probe coated on magnet...
C + R + H: Lane C + R, digested with oligonucleotide-guided RNase H. U: precursor RNA not subjected to circularization conditions. U + H: Lane U, digested with oligonucleotide-guided RNase H. e Sanger sequencing output of RT-PCR across the splice junction of the ...
oligonucleotide synthesis or PCR amplification at guide RNA–target sequence region was 36.5%. This plasmid library was used in the following pooled screening experiments to profile gRNA activity for WT-SpCas9, eSpCas9(1.1), and SpCas9-HF1....