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核酸是脱氧核糖核酸（DNA）和核糖核酸（RNA）的总称，是由许多核苷酸单体聚合成的生物大分子化合物，为生命的最基本物质之一。核酸是一类生物聚合物，是所有已知生命形式必不可少的组成物质，是所有生物分子中最重要的物质，广泛存在于所有动植物细胞、微生物体内。核酸由核苷酸组成，而核苷酸单体由五碳糖、磷酸基和含...
Iodogen-包被的试管可在干燥器中,于室温下保存多年;4.1251标记的抗体,在制备后六周内均可使用(1251的半衰期为59.6天);5.要注意保证所用的Na1251是新鲜的,陈旧制剂的比活性低;6.酪氨酸的碘化偶尔会对抗原的抗体结合位点有干扰,因此降低其结合能力,但这种情况很少见。 抗体的结构:抗体是具有4条多肽链的对称结构,...
Laver JD, Li X, Ray D, Cook KB, Hahn NA, Nabeel-Shah S, Kekis M, Luo H, Marsolais AJ, Fung KY, et al. Brain tumor is a sequence-specific RNA-binding protein that directs maternal mRNA clearance during the Drosophila maternal-to-zygotic transition. Genome Biol. 2015;16:94. Article...
Fluorescent RNAs (FRs), aptamers that bind and activate fluorescent dyes, have been used to image abundant cellular RNA species. However, limitations such as low brightness and limited availability of dye/aptamer combinations with different spectral char
When salt is added, Na+ neutralizes the negative charge of citrate and thus, AuNPs are aggregated. However, the modification of AuNPs with thiolated DNA probes protects nanoparticles (NPs) against salt-induced aggregation due to the formation of a repulsive electric double layer by negative ...
Popravljen je problem zbog toga što su tekstualna polja na portalu koja se koriste za šifrovanje i zbog toga postala nečitljiva. Do ovoga je došlo tokom određenih grešaka pri proveri valjanosti, kao što je greška u veličini priloga da...
The mechanisms by which viruses hijack the genetic machinery of the cells they infect are of current interest. When bacteriophage T4 infects Escherichia coli, it uses three different adenosine diphosphate (ADP)-ribosyltransferases (ARTs) to reprogram
For the preparation of whole cell lysates, HDFs were washed twice with cold PBS and scraped off on ice in lysis buffer (50 mM Tris–HCl, 150 mM NaCl, 1% NP-40, 0.25% Na-deoxycholate, 1 mM EDTA, 100 nM Na3VO4, 1 mM NaF, 10 mM β-glycerophosphate, pH 7.4) from the 6-well pl...