Molecular weight analysis For each protein, the halfway was calculated and corresponded to the value for which the CDF reached 0.5, indicating that 50% of the total protein amount was detected. The apparent MW was obtained using the reference extrapolation (y = 1146.9x2.2577; R2 = 0....
ionic bonds are susceptible to pH changes, which may hinder the sustained release of the loaded biomolecules. Factors such as the number and type of charge groups (for example, primary, secondary and quaternary amino groups and amidine groups) per single polymer can also help determine the ultima...
. Subsequently, a smaller molecular weight EcCas6e protein fused to the ADAR deaminase domain was designed [174]. However, off-target effects have always been an important issue in programmed RNA A-to-I editing; ectopic expression of exogenous ADAR fusion proteins increases the risk of off-targe...
Cells were cultured in 500-mm2 plates until 90% confluence, using a single plate per replicate. Five replicates were performed for the differential sedimentation speed based cell fractionation experiment. Cells were washed twice with PBS and detached using trypsin/EDTA (0.05%) (Thermo Scientific) fo...
Expression levels (FPKM [fragments per kilobase of transcript per million mapped reads]) of all annotated protein-coding genes and lncRNAs were estimated with RSEM (version 1.2.22) (Li and Dewey, 2011). FPKM values as defined by RSEM were added to a pseudo-value of 1e−6 (to avoid ...
(Schuwirthet al., 2005) have provided two structures for this intact ribosome, since there are two independent ribosome assemblies per crystallographic asymmetric unit. There are some significant differences between the two structures, notably that the 30S subunit is rotated about 6° around the so...
蛋白分子量predicted molecular weight: 49kDa 性状Lyophilized or Liquid 免疫原KLH conjugated synthetic peptide derived from human BAT1 亚型IgG 纯化方法affinity purified by Protein A 储存液0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide ...
Indeed, the longer the gene, the higher the read count for a given expression level; thus, any method for which precision is related to read count will tend to report more significant DE statistics for longer genes, even when considering per-base read counts. Hansen et al. [12] ...
and results were recorded in 0 s, 15 s, 1 min, 5 min and 30 min, respectively. Each reaction is repeated independently for three times (n = 3). The band of time 0 s (9mer) serves as internal molecular weight marker: p32-labeled 9mer RNA (5’-AUGGAGAGG-3’)...
In some data sets, the average molecular weight of the isolated RBPs was slightly higher compared to total lysates (Fig.2aleft violin plot). Note that the LC/MS data obtained from cell lysates also appeared to have a similar bias for higher molecular weight proteins, so the slight bias obs...