It has been proposed that the mechanism of action of PTB involves a looping out of exons flanked by pyrimidine tracts. Here, we present Fluorescence, NMR and single molecule data that directly support this mechanism. We show that the RNA binding domains 3 and 4 of PTB (RBD34) bind two ...
1.1 断裂基因存在的证据 R-looping experiment. 将DNA与成熟mRNA(b图红色)杂交,可观察到DNA分子中不能配对而成环的三个内含子区域(A、B、C)。 大多数编码mRNA的基因存在0~362个内含子。大多数内含子比外显子长,内含子占优势是高等真核生物(higher erkaryotes)的显著...
图2. Pol III与Pol II交叉调控的转录干扰(Interference), 成环(Looping)和非编码RNA (noncoding RNA)模型 为了进一步探究这类调控背后的分子机制,研究者首先比较了Pol III在染色质上的结合和其发挥调控作用的这类基因的位置关系,发现Pol III在其调控的这类Pol II转录基因的启动子附近都有特异性结合,但是这些Pol ...
ZBP1 recognition of β-actin zipcode induces RNA looping. ZBP1 (zipcode-binding protein 1) was originally discovered as a trans-acting factor for the "zipcode" in the 3′ untranslated region (UTR) of the β-actin ... - 《Genes & Development》 被引量: 0发表: 2010年 Different motif ...
This feature of lncRNAs enables such biological phenomena as gene imprinting, dosage compensation of sex chromosomes, transcriptional enhancement, chromosome looping and antisense regulation.Abstract Long non-coding RNAs (lncRNAs) are a diverse class of RNAs that engage in numerous biological processes ...
CTCF-mediated chromatin looping A process by which CTCF binding to DNA facilitates long-range chromatin interactions between regions that are far apart on the linear chromosome. RNA–DNA triplex A form of RNA–DNA interaction in which the RNA strand inserts itself into the major groove of the du...
This structural signature is consistent with RNA looping around MBNL proteins such that different zinc fingers interact with different GCs. RNA looping as a mechanism of RNA recognition has been proposed for PTB (Oberstrass et al., 2005, Pérez et al., 1997) and is also consistent with the ...
RNA合101120 RNA的生物合成(转录)以一条DNA的片段为模板，由RNA聚合酶催化合成RNA，使遗传信息由DNA流向RNA的过程 转录：（DDRP）DNA→RNA 转录与复制相同点 模板都为DNA都需依赖DNA的聚合酶都形成磷酸二酯键 方向都为5’→3’都遵循碱基互补配对原则 转录与复制不同点 复制原料dNTP（ATCG）转录NTP(AUCG)模板 ...
第一节RNA加工概述 一、断裂基因（Splitgenes）（一）定义 MosteukaryoticcodingregionofgenesareinterruptedbynoncodingDNA.Thecodingregions,orexpressedregionsofgenesarecalledexons(外显子).Thenoncodingregionsofgenesarecalledintrons(内含子).（二）断裂基因的证据 R-looping Theintronregions...
You might find these chapters and articles relevant to this topic. Chapter Biochemistry Nucleus inheritance. Within the nucleus, one or more mobileare present; they contain ribonucleic acid (RNA), and are involved in cell.is a precursor of cytoplasmic(seeCh. 1). ...