RNA-splicing ligase RtcB (strain K12) 来源宿主 兔 反应性 大肠杆菌 保存建议 建议收到该RNA-splicing ligase RtcB (strain K12) Antibody (HRP)产品后,储存温度为-20°C。避免重复冻融 其他 Abbexa总部位于英国剑桥的剑桥科学园,是一家专业的全球抗体供应商和蛋白质供应商,致力于为生命科学、药物开发和生物技...
Here, we describe a novel in vivo model in Caenorhabditis elegans for the conserved RNA ligase RtcB and show that RtcB ligates the xbp-1 mRNA during the IRE-1 branch of the unfolded protein response. Without RtcB, protein stress results in the accumulation of unligated xbp-1 mRNA fragments ...
2. 基于核酶构建的环状RNA过表达载体:核酶(ribozyme)是指具有催化功能的小分子RNA[27],可以降解特异的RNA序列。自剪切核酶一般可以产生5'端的游离羟基和3'端的2',3'-环磷酸酯结构,RtcB可以将其连接。Jaffrey[28]等在2015年就曾经报道设计了基于tRNA的环状RNA过表达体系(tricY),tRNA加工成熟的过程中内含子区域可...
T4 RNA Ligase 2, truncated KQ M0373L 10,000 units ¥2,929 ¥2,197 M0373S 2,000 units ¥739 ¥555 RtcB Ligase M0458S 25 rxns ¥829 ¥622 SplintR Ligase M0375L 6,250 units ¥4,909 ¥3,682 M0375S 1,250 units ¥1,099 ¥825 Thermostable 5´ AppDNA/RNA ...
The 3′-phosphate of the spliced RNA is converted into 2′,3′-cyclic phosphate by Rtca, whereas the RNA ligase Rtcb reverses this reaction and re-ligates the processed RNA, with the help of its catalyst Archease. Thus Rtca and Archease may have opposing functions on RNA repair/splicing ...
RNA ligase activity of the recombinant RtcB proteins fromP. aerophilumandE. colistrictly depends on the presence of bivalent metal ions [102,104]. Studies probing the active sites of archaeal and bacterial RtcB by mutagenesis [102,104,105] could in part confirm early predictions concerning essentia...
“Twister” aptamers to generate a 2’, 3’-cyclic phosphate group at the 5’ terminus.133Subsequently, the ubiquitous endogenous RNA ligase RtcB connects the 3’ terminus and 5’-OH.134,135It is worth mentioning that these methods involve sophisticated steps and are not suitable for the ...
Structural and mechanistic insights into activation of the human RNA ligase RTCB by Archease RNA ligases of the RTCB-type play an essential role in tRNA splicing, the unfolded protein response and RNA repair. RTCB is the catalytic subunit of the pe... JL Gerber,SI Morales Guzmán,L Worf,.....
RNA plays a multitude of cellular roles in all kingdoms of life and is a promising area of research in biotechnology and therapeutic development. RNA is an essential carrier of genetic information that can catalyze chemical reactions and influence gene expression. Our understanding of RNAs as regulat...
ATP-dependent RNA ligation involves a series of nucleotidyl transfer steps similar to those that occur during ligation via the RtcB pathway, with the ligase reacting with ATP to form a covalent ligase-AMP, followed by transfer of the AMP to the 5′-PO4 end of the RNA to form an AppRNA...