The RNA-dependentRNApolymeraseis responsible for genome replication ofRNA viruses. Nuclear magnetic resonance experiments and molecular dynamics simulations have indicated that efficient and faithful polymerase function requires highly coordinated internal protein motions. Interference with these motions, either th...
笔者联想到曾经看到过的一个研究 (8):Farias等人通过祖先序列重建推测了祖先tRNA的序列,然后将祖先tRNA序列按照mRNA翻译的规律翻译为多肽链,预测该多肽链的结构,并与已知的所有蛋白质结构做比对,发现最相似的竟然是RNA依赖的RNA聚合酶(RNA-dependent RNA Polymerase)。该类聚合酶隶属右手聚合酶,在病毒中分布很广泛,而...
2023年5月16日，来自美国纽约大学医学院的Evgeny Nudler团队在Cell杂志发表了文章RNA polymerase drives ribonucleotide excision DNA repair in E. coli，他们通过冷冻电镜解析了RER与携带错误插入rNMPs的DNA双链的复合物结构，分析了RER对错误插入rNMPs的修复机制。研究人员首先在大肠杆菌中联合高亲和pull-down以及定量质...
2023年5月16日,来自美国纽约大学医学院的Evgeny Nudler团队在Cell杂志发表了文章RNA polymerase drives ribonucleotide excision DNA repair in E. coli,他们通过冷冻电镜解析了RER与携带错误插入rNMPs的DNA双链的复合物结构,分析了RER对错误插入rNMPs的修复机制。 研...
而病毒生长复制所须的核酸聚合酵素活性(RNA dependent RNA polymerase)则被发现存在於NS5。对於其它分子量较小的亲脂 … www.nics.org.tw|基于2个网页 2. 多聚酶 RdRP:RNA 依赖性RNA多聚酶(RNA dependent RNA polymerase) , 作用是以siRNA 为一种特殊的引物,以靶mRNA为模 … ...
以rna为模板的rna聚合酶，就是rna复制酶，一般rna病毒才会编码有这种酶
切割依赖性/非依赖性解旋(slicer-dependent/independent unwinding)机制为:miRNA duplex在ATP作用下装载到AGO蛋白上,形成前体RISC(pre-RISC),pre-RISC不具有转录后调控功能[26,27,28]。当引导链和过客链高度互补时,AGO2蛋白在Mg2+参与下切割过客链中特定核苷酸间的磷酸二酯键[26],且Mg2+浓度越高,AGO2切割活力越...
5. Weinmann,R. & Roeder, R. G. Role of DNA-dependent RNA polymerase 3 in thetranscription of the tRNA and 5S RNA genes.Proc Natl Acad Sci U S A71,1790-1794, doi:10.1073/pnas.71.5.1790 (1974). 6. Weinmann,R., Raskas, H. J. & Roeder, R. G. Role of DNA-dependent RNA polym...
The widespread nature of several viruses is greatly credited to their rapidly altering RNA genomes that enable the infection to persist despite challenges presented by host cells. Within the RNA genome of infections is RNA-dependent RNA polymerase (RdRp), which is an essential enzyme that helps in...
5. Weinmann,R. & Roeder, R. G. Role of DNA-dependent RNA polymerase 3 in thetranscription of the tRNA and 5S RNA genes.Proc Natl Acad Sci U S A71,1790-1794, doi:10.1073/pnas.71.5.1790 (1974). 6. Weinmann,R...