The HuProt Human proteome microarray contains over 21,000 GST-purified unique recombinant human proteins in yeast, including >81% of the canonically expressed proteins as defined by the Human Protein Atlas43,44. The proteins in the HuProt protein microarray are folded in their native conformation. ...
we suggest that some of 545 proteins unique to the R-DeeP dataset could be RDPs or unknown RBPs. Among these proteins, we identified 181 uncharacterized proteins of which 138 are annotated asPlasmodiumspecific. This cluster also contains seven heptatricopeptide repeat (HPR) proteins...
DNA:DNA:RNA triplexes that are formed through Hoogsteen base-pairing of the RNA in the major groove of the DNA duplex have been observed in vitro, but the extent to which these interactions occur in cells and how they impact cellular functions remains elusive. Using a combination of bioinforma...
To test the RNA-guided endonuclease activity of Fz, we focused on SpuFz1, given its larger REC domain relative to Fz2. SpuFz1 is encoded in a roughly 2.1-kilobase pair (kbp) locus containing a single open reading frame (ORF) flanked by well-conserved, non-degraded transposon inverted repe...
The initial transcript of eukaryotic genes contains several subregions (seeFig. 1.4). Most striking is the tandem alignment of exons and introns. Precise excision of intron sequences and ligation of exons is critical for production ofmature mRNA. This process is calledmRNA splicing, and it occurs...
Analyses of the DNA sequence of WRAP53 using the Ensemble database (https://useast.ensembl.org/index.html) revealed that its 5’UTR contains an APA (AATAAA) site and m6A sites (RRACH) upstream of the start codon (Fig.5A). Generally, the transcribed mRNA is cleaved at the APA sites ...
The group of patients for this study was formed using a database of patients with a high degree of obesity undergoing treatment of bariatric surgery at the Federal State Budgetary Institution “V.A. Almazov National Medical Research Centre” of the Ministry of Health of the Russian Federation, ...
and 2), but that only the dengue helicase contains domain 3, indicating that compounds that inhibit the viral helicase are likely to be specific to the this enzyme. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of the ...
Illumina's Genome Analyzer II high-throughput sequencing system was used to sequence RNA from Saccharomyces cerevisiae grown in three different media: standard YP Glucose (YPD, a rich medium), Delft Glucose (Del, a minimal medium), and YP Glycerol (Gly, which contains a non-fermentable carbon ...
UNK contains a region with a distinct heptad repeat pattern of a coiled-coil motif (residues 643–767; Supplementary Fig. 5A). To investigate the possible structural role of this motif, we used AlphaFold to generate structure predictions for UNKFULL and a C-terminal fragment, residues 637–810...