Using quantitative real-time polymerase chain reaction (RT-PCR), we found that transient RIPK2 overexpression in PC3 cells, RIPK2-KO in 22Rv1 cells, or RIPK2 inhibition by GSK583 (a RIPK2-selective inhibitor) at different doses and time only modestly (~10%) regulated MYC mRNA abundance (Sup...
However, no data on any clinical evaluations are currently available. As for RIPK3, diverse isoform-specific inhibitors have been characterized, such as the pioneering compounds GSK843 (5) and GSK872 (6) [34]. Later, the inhibitor HS-1371 (7) [35] as well as other compounds have been ...
lysates were performed by lysing cells in a 10× RIPA buffer containing 20 mM Tris-HCl (pH 7.5) 150 mM NaCl, 1 mM Na2 EDTA 1 mM EGTA 1% NP-40 1% sodium deoxycholate 2.5 mM sodium pyrophosphate 1 mM β-glycerophosphate 1 mM Na3 VO4 1 µg/mL leupeptin and protease inhibitor mixture...