Purified naive CD4T cells (CD4+, CD45RA+) were purified using a kit for isolation of naive CD4T cells (Mylteni Biotec Inc.) and the AutoMACS sorter. The purity (CD4+, CD45RA+) exceeded 95%. To prepare moDCs, all PBMCs were allowed to adhere to a culture flask at 37° C. for...
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