mounted, coverslipped and stored at 4 °C. A more detailed immunostaining protocol is available per request. The third series of sections were collected and kept for long-term storage in vials containing 2% DMSO and 20% glycerol in phosphate buffer (PB) at − 20 °C. Using a d...
The pellet was resuspended in TN buffer (25 mM Tris-HCl, pH 7.8, 150 mM NaCl, 1 mM DTT, protease inhibitors, 10% sucrose) containing 1% Triton X-100 all at + 4 °C and both the pellet and the supernatant (the equivalent to 1 mg of total mitochondrial protein) were ...
download if a fragment is unavailable (Alias: --no-skip-unavailable-fragments) --keep-fragments Keep downloaded fragments on disk after downloading is finished --no-keep-fragments Delete downloaded fragments after downloading is finished (default) --buffer-size SIZE Size of download buffer, e.g....
The beads were subjected to IB after three-time washes with lysis buffer. Representative data from three independent experiments are shown. (E) PGAM5 dephosphorylates SRm160 in vitro. Flag-SRm160 transiently expressed in HeLa cells was immunoprecipitated with the Flag antibody. The precipitates were...
ID3DBlob::GetBufferSize method (Windows) HTTP_REQUEST structure (Windows) EnumLanguageGroupsProc callback function (Windows) EnumTimeFormatsProc callback function (Windows) SecureZeroMemory function (Windows) WLAN_NOTIFICATION_DATA structure (Windows) ConnectionStatusHandler function (Windows) UserName (Wi...
Mouse tail or ear biopsies were incubated in 1.5 ml tubes for 30 min in 40 µl lysis buffer (25 mM NaOH, 0.2 mM EDTA, pH 12) at 95 °C under mild agitation. Precipitate was spun down at 15,000 rpm for 30 s, 40 µl of neutralizing buffer (40 mM Tris/HCl pH 5.0) were ad...
It is commonly believed that glutathione and metallothioneins –a family of metal-binding, cysteine rich proteins – are imperative to astrocytes’ ability to buffer Cu [57]. To gain insight into the formation mechanism and biological significance of Cu-rich aggregates, it would be beneficial to ...
Using the area within each circular buffer we calculated the proportion of land cover surrounding each site including building footprints, road surface area, and land cover. Polygons for building footprints and roads were generated using polyline data from a 2008 to 2009 survey obtained from the ...
If FALSE, the search direction is toward the beginning of the buffer.Return ValueNonzero if the lpszFind text is found; otherwise 0.ExampleSee the example for CRichEditView::FindText.CRichEditView::GetCharFormatSelectionCall this function to get the character formatting attributes of the ...
Natural oxidases mainly rely on cofactors and well-arranged amino acid residues for catalysing electron-transfer reactions but suffer from non-recovery of their activity upon externally induced protein unfolding. However, it remains unknown whether resid